ArticleThe Journal of biological chemistry2008
Control of translocation through the Sec61 translocon by nascent polypeptide structure within the ribosome.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- A genome-wide association study identifies a novel East Asian-specific locus for dementia with Lewy bodies in Japanese subjects.Molecular medicine (Cambridge, Mass.) · 2025Article
- Methods to study folding of alpha-helical membrane proteins in lipids.Open biology · 2022Review
- CFTR trafficking mutations disrupt cotranslational protein folding by targeting biosynthetic intermediates.Nature communications · 2020Article
- Cell-free expression tools to study co-translational folding of alpha helical membrane transporters.Scientific reports · 2020Article
- Oncoprotein AEG-1 is an endoplasmic reticulum RNA-binding protein whose interactome is enriched in organelle resident protein-encoding mRNAs.RNA (New York, N.Y.) · 2018Article
- The Sec61/SecY complex is inherently deficient in translocating intrinsically disordered proteins.The Journal of biological chemistry · 2017Article
- Determinants of Helix Formation for a Kv1.3 Transmembrane Segment inside the Ribosome Exit Tunnel.Journal of molecular biology · 2017Article
- The Ribosome-Sec61 Translocon Complex Forms a Cytosolically Restricted Environment for Early Polytopic Membrane Protein Folding.The Journal of biological chemistry · 2015Article
- Mechanisms of integral membrane protein insertion and folding.Journal of molecular biology · 2015Review
- The α-helical structure of prodomains promotes translocation of intrinsically disordered neuropeptide hormones into the endoplasmic reticulum.The Journal of biological chemistry · 2013Article
- Structural features within the nascent chain regulate alternative targeting of secretory proteins to mitochondria.The EMBO journal · 2013Article
- Kinetic analysis of ribosome-bound fluorescent proteins reveals an early, stable, cotranslational folding intermediate.The Journal of biological chemistry · 2012Article
- Polytopic membrane protein folding at L17 in the ribosome tunnel initiates cyclical changes at the translocon.The Journal of cell biology · 2011Article
- Nascent peptide side chains induce rearrangements in distinct locations of the ribosomal tunnel.Journal of molecular biology · 2011Article
- Protein folding and quality control in the endoplasmic reticulum: Recent lessons from yeast and mammalian cell systems.Current opinion in cell biology · 2011Review
- Protein folding in the cell: challenges and progress.Current opinion in structural biology · 2011Review
- Lateral opening of a translocon upon entry of protein suggests the mechanism of insertion into membranes.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- In vitro incorporation of nonnatural amino acids into protein using tRNA(Cys)-derived opal, ochre, and amber suppressor tRNAs.RNA (New York, N.Y.) · 2010Article
- Positive charges of translocating polypeptide chain retrieve an upstream marginal hydrophobic segment from the endoplasmic reticulum lumen to the translocon.Molecular biology of the cell · 2010Article
- Membrane insertion and biogenesis of the Turnip crinkle virus p9 movement protein.Journal of virology · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
During polytopic protein biogenesis, multiple transmembrane segments (TMs) must pass through the ribosome exit tunnel and into the Sec61 translocon prior to insertion into the endoplasmic reticulum membrane. To investigate how movement of a newly synthesized TM along this integration pathway might be influenced by synthesis of a second TM, we used photocross-linking probes to detect the proximity of ribosome-bound nascent polypeptides to Sec61alpha. Probes were inserted at sequential sites within TM2 of the aquaporin-1 water channel by in vitro translation of truncated mRNAs. TM2 first contacted Sec61alpha when the probe was positioned approximately 38 residues from the ribosome peptidyltransferase center, and TM2-Sec61alpha photoadducts decreased markedly when the probe was >80 residues from the peptidyltransferase center. Unexpectedly, as nascent chain length was gradually extended, photocross-linking at multiple sites within TM2 abruptly and transiently decreased, indicating that TM2 initially entered, withdrew, and then re-entered Sec61alpha. This brief reduction in TM2 photocross-linking coincided with TM3 synthesis. Replacement of TM3 with a secretory reporter domain or introduction of proline residues into TM3 changed the TM2 cross-linking profile and this biphasic behavior. These findings demonstrate that the primary and likely secondary structure of the nascent polypeptide within the ribosome exit tunnel can influence the timing with which topogenic determinants contact, enter, and pass through the translocon.
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