ArticleJournal of the American Society for Mass Spectrometry2008
Development of an automated digestion and droplet deposition microfluidic chip for MALDI-TOF MS.
Article in Journal of the American Society for Mass Spectrometry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 62 citations in OpenAlex.
- Accelerated protein digestion by numbering-up nanochannels, followed by LC-MS analysis.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2026Article
- Advances in Chemical and Biological Methods to Identify Microorganisms-From Past to Present.Microorganisms · 2019Review
- Microfluidic Platform with In-Chip Electrophoresis Coupled to Mass Spectrometry for Monitoring Neurochemical Release from Nerve Cells.Analytical chemistry · 2016Article
- Immobilization of lambda exonuclease onto polymer micropillar arrays for the solid-phase digestion of dsDNAs.Analytical chemistry · 2014Article
- MALDI-target integrated platform for affinity-captured protein digestion.Analytica chimica acta · 2014Article
- Microfluidic LC device with orthogonal sample extraction for on-chip MALDI-MS detection.Lab on a chip · 2013Article
- Label-free quantitation of peptide release from neurons in a microfluidic device with mass spectrometry imaging.Lab on a chip · 2012Article
- Incubated protein reduction and digestion on an electrowetting-on-dielectric digital microfluidic chip for MALDI-MS.Analytical chemistry · 2010Article
- Print-and-peel fabrication for microfluidics: what's in it for biomedical applications?Annals of biomedical engineering · 2010Review
- Recent advances in microfluidic detection systems.Bioanalysis · 2009Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An automated proteolytic digestion bioreactor and droplet deposition system was constructed with a plastic microfluidic device for off-line interfacing to matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The microfluidic chips were fabricated in poly(methyl methacrylate) (PMMA), using a micromilling machine and incorporated a bioreactor, which was 100 microm wide, 100 microm deep, and possessed a 4 cm effective channel length (400 nL volume). The chip was operated by pressure-driven flow and mounted on a robotic fraction collector system. The PMMA bioreactor contained surface immobilized trypsin, which was covalently attached to the UV-modified PMMA surface using coupling reagents N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and hydroxysulfosuccinimide (sulfo-NHS). The digested peptides were mixed with a MALDI matrix on-chip and deposited as discrete spots on MALDI targets. The bioreactor provided efficient digestion of a test protein, cytochrome c, at a flow rate of 1 microL/min, producing a reaction time of approximately 24 s to give adequate sequence coverage for protein identification. Other proteins were also evaluated using this solid-phase bioreactor. The efficiency of digestion was evaluated by monitoring the sequence coverage, which was 64%, 35%, 58%, and 47% for cytochrome c, bovine serum albumin (BSA), myoglobin, and phosphorylase b, respectively.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.