ArticleMicrobial pathogenesis2008
Verification and dissection of the ospC operator by using flaB promoter as a reporter in Borrelia burgdorferi.
Article in Microbial pathogenesis, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- The Lyme disease spirochete, Borrelia burgdorferi, as a model vector-borne pathogen: insights on regulation of gene and protein expression.Current opinion in microbiology · 2023Review
- Gac Is a Transcriptional Repressor of the Lyme Disease Spirochete's OspC Virulence-Associated Surface Protein.Journal of bacteriology · 2023Article
- The Consistent Tick-Vertebrate Infectious Cycle of the Lyme Disease Spirochete Enables Borrelia burgdorferi To Control Protein Expression by Monitoring Its Physiological Status.Journal of bacteriology · 2022Review
- Transcriptomic insights on the virulence-controlling CsrA, BadR, RpoN, and RpoS regulatory networks in the Lyme disease spirochete.PloS one · 2018Article
- Multiple and Diverse vsp and vlp Sequences in Borrelia miyamotoi, a Hard Tick-Borne Zoonotic Pathogen.PloS one · 2016Article
- Article
- Regulatory protein BBD18 of the lyme disease spirochete: essential role during tick acquisition?mBio · 2014Article
- Article
- Regulation of the virulence determinant OspC by bbd18 on linear plasmid lp17 of Borrelia burgdorferi.Journal of bacteriology · 2011Article
- Adhesion mechanisms of Borrelia burgdorferi.Advances in experimental medicine and biology · 2011Review
- lacZ reporter system for use in Borrelia burgdorferi.Applied and environmental microbiology · 2010Article
- Article
- Role of the surface lipoprotein BBA07 in the enzootic cycle of Borrelia burgdorferi.Infection and immunity · 2010Article
- Characterization of the highly regulated antigen BBA05 in the enzootic cycle of Borrelia burgdorferi.Infection and immunity · 2010Article
- GuaA and GuaB are essential for Borrelia burgdorferi survival in the tick-mouse infection cycle.Journal of bacteriology · 2009Article
- The Borrelia burgdorferi flaB promoter has an extended -10 element and includes a T-rich -35/-10 spacer sequence that is essential for optimal activity.FEMS microbiology letters · 2009Article
- Abrogation of ospAB constitutively activates the Rrp2-RpoN-RpoS pathway (sigmaN-sigmaS cascade) in Borrelia burgdorferi.Molecular microbiology · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Lyme disease spirochete Borrelia burgdorferi must repress expression of outer surface protein C (OspC) to effectively evade specific humoral immunity and to establish persistent infection. This ability largely relies upon a regulatory element, the only operator that has been reported in spirochetal bacteria. Immediately upstream of the ospC promoter, two sets of inverted repeats (IRs) constitute small and large palindromes, in which the right IR of the large palindrome contains the left IR of the small one, and may collectively function as the ospC operator. In the study, the large palindrome with or without the small IR was fused with an flaB promoter, which was used to drive expression of a promoterless ospC copy as a reporter gene, and introduced into OspC-deficient B. burgdorferi. The presence of the large palindrome alone significantly reduced ospC expression driven by the fused flaB promoter in the joint tissue of severe combined immunodeficiency (SCID) mice, and rescued spirochetes from elimination by passively transferred OspC antibody in infected SCID mice and specific immune responses elicited in immunocompetent mice, confirming a function of the IRs as an operator. Inclusion of the small IR further enhanced the ability of the large palindrome to reduce the activity of the fused flaB promoter, indicating that the small IR is a part of the operator. Taken together, the study led to successful verification and dissection of the ospC operator.
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