Evidence map›Paper›PMID 18479828›Full record

ArticleNeuroscience2008

Colocalization of neurokinin-1, N-methyl-D-aspartate, and AMPA receptors on neurons of the rat nucleus tractus solitarii.

L H Lin, O M Taktakishvili, W T Talman

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuroscience, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
0.8field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 36 citations in OpenAlex.

  1. Intrinsic organization of the corpus callosum.Frontiers in physiology · 2024
    Review
  2. Article
  3. Article
  4. Interactions between Brainstem Neurons That Regulate the Motility to the Stomach.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Astrocytes in the rat nucleus tractus solitarii are critical for cardiovascular reflex control.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013
    Article
  13. Review
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Cardiac damage after lesions of the nucleus tractus solitarii.American journal of physiology. Regulatory, integrative and comparative physiology · 2009
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

L H LinDepartment of Neurology, University of Iowa, 1191 Medical Laboratories, Iowa City, IA 52242, USA. li-hsien-lin@uiowa.edu
O M Taktakishvili
W T Talman
University of Iowa · US

Funding

CARDIOVASCULAR RESPONSE TO GLUTAMATE NITRIC OXIDER01HL059593 · NHLBI · UNIVERSITY OF IOWA · PI TALMAN, WILLIAM TEMPLE · 1999 to 2006
$1.4M
NHLBI NIH HHS R01 HL059593NHLBI NIH HHS R01 HL59593
6 · The paper itself

Abstract

Substance P (SP) and glutamate are implicated in cardiovascular regulation by the nucleus tractus solitarii (NTS). Our earlier studies suggest that SP, which acts at neurokinin 1 (NK1) receptors, is not a baroreflex transmitter while glutamate is. On the other hand, our recent studies showed that loss of NTS neurons expressing NK1 receptors leads to loss of baroreflex responses and increased blood pressure lability. Furthermore, studies have suggested that SP may interact with glutamate in the NTS. In this study, we sought to test the hypothesis that NK1 receptors colocalize with glutamate receptors, either N-methyl-d-aspartate (NMDA) receptors or AMPA receptors or both in the NTS. We performed double-label immunofluorescent staining for NK1 receptors and either N-methyl-d-aspartate receptor subunit 1 (NMDAR1) or AMPA specific glutamate receptor subunit 2 (GluR2) in the rat NTS. Because vesicular glutamate transporter 2 (VGLUT2) containing fibers are prominent in portions of the NTS where cardiovascular afferent fibers terminate, we also performed double-label immunofluorescent staining for NK1 receptors and VGLUT2. Confocal microscopic images showed that NK1 receptors-immunoreactivity (IR) and NMDAR1-IR colocalized in the same neurons in many NTS subnuclei. Almost all NTS neurons positive for NK1 receptor-IR also contained NMDAR1-IR, but only 53.4% to 74.8% of NMDAR1-IR positive neurons contained NK1 receptors-IR. NK1 receptor-IR and GluR2-IR also colocalized in many neurons in NTS subnuclei. A majority of NK1 receptor-IR positive NTS neurons also contained GluR2-IR, but only 45.8% to 73.9% of GluR2-IR positive NTS neurons contained NK1 receptors-IR. Our results also showed that fibers labeled for VGLUT2-IR were in close apposition to fibers and neurons labeled for NK1 receptor-IR. The data support our hypothesis, provide an anatomical framework for glutamate and SP interactions, and may explain the loss of baroreflexes when NTS neurons, which could respond to glutamate as well as SP, are killed.

Indexed as

AnimalsFluorescent Antibody Technique, IndirectMaleMicroscopy, ConfocalNeuronsRatsRats, Sprague-DawleyReceptors, AMPAReceptors, Neurokinin-1Receptors, N-Methyl-D-AspartateSolitary NucleusVesicular Glutamate Transport Protein 2glutamate receptor ionotropic, AMPA 2Receptors, AMPAReceptors, Neurokinin-1Receptors, N-Methyl-D-AspartateSlc17a6 protein, ratVesicular Glutamate Transport Protein 2

Identifiers

PMID18479828
PMCPMC2587108
OpenAlexW2055506669

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.