ArticleJournal of the American Chemical Society2008
Microscale NMR screening of new detergents for membrane protein structural biology.
Article in Journal of the American Chemical Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
- Hydrogen Bonding within DynamicJournal of the American Chemical Society · 2026Article
- Improved Pendant-Bearing Glucose-Neopentyl Glycols for Membrane Protein Stability.Bioconjugate chemistry · 2025Article
- Lipid Membrane Mimetics in Functional and Structural Studies of Integral Membrane Proteins.Membranes · 2021Review
- Solution NMR: A powerful tool for structural and functional studies of membrane proteins in reconstituted environments.The Journal of biological chemistry · 2019Review
- Perturbations of Native Membrane Protein Structure in Alkyl Phosphocholine Detergents: A Critical Assessment of NMR and Biophysical Studies.Chemical reviews · 2018Review
- Micro-scale NMR Experiments for Monitoring the Optimization of Membrane Protein Solutions for Structural Biology.Bio-protocol · 2015Article
- NMR polypeptide backbone conformation of the E. coli outer membrane protein W.Structure (London, England : 1993) · 2014Article
- Solution-NMR characterization of outer-membrane protein A from E. coli in lipid bilayer nanodiscs and detergent micelles.Chembiochem : a European journal of chemical biology · 2014Article
- Micro-coil NMR to monitor optimization of the reconstitution conditions for the integral membrane protein OmpW in detergent micelles.Journal of biomolecular NMR · 2012Article
- Translational diffusion measurements by microcoil NMR in aqueous solutions of the Fos-10 detergent-solubilized membrane protein OmpX.The journal of physical chemistry. B · 2012Article
- New amphiphiles for membrane protein structural biology.Methods (San Diego, Calif.) · 2011Review
- The high-throughput protein sample production platform of the Northeast Structural Genomics Consortium.Journal of structural biology · 2010Article
- Design, synthesis, and properties of branch-chained maltoside detergents for stabilization and crystallization of integral membrane proteins: human connexin 26.Langmuir : the ACS journal of surfaces and colloids · 2010Article
- An Escherichia coli-based cell-free system for large-scale production of functional mammalian membrane proteins suitable for X-ray crystallography.Journal of molecular microbiology and biotechnology · 2010Article
- A microscale protein NMR sample screening pipeline.Journal of biomolecular NMR · 2010Article
- Tripod Amphiphiles for Membrane Protein Manipulation.Molecular bioSystems · 2010Article
- Structural and dynamic properties of juxta-membrane segments of caveolin-1 and caveolin-2 at the membrane interface.European biophysics journal : EBJ · 2010Article
- NMR characterization of membrane protein-detergent micelle solutions by use of microcoil equipment.Journal of the American Chemical Society · 2009Article
- Production of membrane proteins for NMR studies using the condensed single protein (cSPP) production system.Journal of structural and functional genomics · 2009Article
- Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins.Progress in nuclear magnetic resonance spectroscopy · 2009Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The rate limiting step in biophysical characterization of membrane proteins is often the availability of suitable amounts of protein material. It was therefore of interest to demonstrate that microcoil nuclear magnetic resonance (NMR) technology can be used to screen microscale quantities of membrane proteins for proper folding in samples destined for structural studies. Micoscale NMR was then used to screen a series of newly designed zwitterionic phosphocholine detergents for their ability to reconstitute membrane proteins, using the previously well characterized beta-barrel E. coli outer membrane protein OmpX as a test case. Fold screening was thus achieved with microgram amounts of uniformly (2)H, (15)N-labeld OmpX and affordable amounts of the detergents, and prescreening with SDS-gel electrophoresis ensured efficient selection of the targets for NMR studies. A systematic approach to optimize the phosphocholine motif for membrane protein refolding led to the identification of two new detergents, 138-Fos and 179-Fos, that yield 2D [ (15)N, (1)H]-TROSY correlation NMR spectra of natively folded reconstituted OmpX.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.