ArticleJournal of cell science2008
Identification of a novel N-terminal hydrophobic sequence that targets proteins to lipid droplets.
Article in Journal of cell science, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
What it found
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Who cites it
76 citing papers in PubMed, 114 citations in OpenAlex.
- Mechanisms influencing transient cytoplasmic protein targeting to intracellular lipid droplets.Biochemical Society transactions · 2026Review
- TMT1A inhibits lung adenocarcinoma progression by suppressing M2 macrophage polarization.iScience · 2026Article
- Epigenetic priming promotes tyrosine kinase inhibitor resistance and oncogene amplification.Nature structural & molecular biology · 2026Article
- GBA1 Gene-Associated Transcriptomic Signatures Reveal Risk Genes in Parkinson's Disease.Biomedicines · 2025Article
- Navigating lipid droplet proteins - part I: ER targeting routes of lipid droplet-destined membrane proteins.Biochemical Society transactions · 2025Review
- Expression pattern, subcellular localization ofApplied and environmental microbiology · 2025Article
- METTL7A improves bovine IVF embryo competence by attenuating oxidative stress†.Biology of reproduction · 2025Article
- Proximity proteomics reveals a mechanism of fatty acid transfer at lipid droplet-mitochondria- endoplasmic reticulum contact sites.Nature communications · 2025Article
- Integrative proteomic and lipidomic analysis of GNB1 and SCARB2 knockdown in human subcutaneous adipocytes.PloS one · 2025Article
- METTL7A improves bovine IVF embryo competence by attenuating oxidative stress.bioRxiv : the preprint server for biology · 2024Article
- Hairpin protein partitioning from the ER to lipid droplets involves major structural rearrangements.Nature communications · 2024Article
- Identification of T-cell exhaustion-related genes and prediction of their immunotherapeutic role in lung adenocarcinoma.Journal of Cancer · 2024Article
- ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover.Protein & cell · 2023Article
- METTL7A (TMT1A) and METTL7B (TMT1B) Are Responsible for AlkylDrug metabolism and disposition: the biological fate of chemicals · 2023Article
- A Novel Localization of METTL7A in Bergmann Glial Cells in Human Cerebellum.International journal of molecular sciences · 2023Article
- Evidence Based on an Integrative Analysis of Multi-Omics Data on METTL7A as a Molecular Marker in Pan-Cancer.Biomolecules · 2023Article
- LncRNA EPR-induced METTL7A1 modulates target gene translation.Nucleic acids research · 2022Article
- Recent progress in strategies for steroid production in yeasts.World journal of microbiology & biotechnology · 2022Review
- Co-translational biogenesis of lipid droplet integral membrane proteins.Journal of cell science · 2022Article
- Induction of mJournal of experimental & clinical cancer research : CR · 2022Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
AAM-B is a putative methyltransferase that is a resident protein of lipid droplets. We have identified an N-terminal 28 amino acid hydrophobic sequence that is necessary and sufficient for targeting the protein to droplets. This sequence will also insert AAM-B into the endoplasmic reticulum (ER). A similar hydrophobic sequence (1-23) in the cytochrome p450 2C9 cannot substitute for 1-28 and only inserts AAM-B into the ER, which indicates that hydrophobicity and ER anchoring are not sufficient to reach the droplet. We found that a similar N-terminal hydrophobic sequence in cytochrome b5 reductase 3 and ALDI could also heterologously target proteins to droplets. Targeting is not affected by changing a conserved proline residue that potentially facilitates the formation of a hairpin loop to leucine. By contrast, targeting is blocked when AAM-B amino acids 59-64 or 65-70, situated downstream of the hydrophobic sequence, are changed to alanines. AAM-B-GFP expressed in Saccharomyces cerevisiae is also faithfully targeted to lipid bodies, indicating that the targeting mechanism is evolutionarily conserved. In conclusion, a class of hydrophobic sequences exists that when placed at the N-terminus of a protein will cause it to accumulate in droplets and in the ER.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.