ArticleThe Journal of biological chemistry2008
Prothyrotropin-releasing hormone targets its processing products to different vesicles of the secretory pathway.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Signal Amplification in the HPT Axis-Evidence for Its Existence, Location, Significance, and Molecular Mechanisms.Acta physiologica (Oxford, England) · 2026Review
- IGF-like growth factors that couple food sensing to developmental decisions employ different release mechanisms in a single pair ofbioRxiv : the preprint server for biology · 2026Article
- Amyloid Protein-Induced Remodeling of Morphometry and Nanomechanics in Human Platelets.Biomedicines · 2025Article
- ProSAAS neuropeptides and receptors GPR171 and GPR83: Potential therapeutic applications for pain, anxiety, and body weight regulation.The Journal of pharmacology and experimental therapeutics · 2025Review
- Ghrelin Action in the PVH of Male Mice: Accessibility, Neuronal Targets, and CRH Neurons Activation.Endocrinology · 2023Article
- Morphometric and Nanomechanical Screening of Peripheral Blood Cells with Atomic Force Microscopy for Label-Free Assessment of Alzheimer's Disease, Parkinson's Disease, and Amyotrophic Lateral Sclerosis.International journal of molecular sciences · 2023Review
- Cell-specific secretory granule sorting mechanisms: the role of MAGEL2 and retromer in hypothalamic regulated secretion.Frontiers in cell and developmental biology · 2023Review
- Platelets' Nanomechanics and Morphology in Neurodegenerative Pathologies.Biomedicines · 2022Article
- Platelets in Neurodegenerative Conditions-Friend or Foe?Frontiers in immunology · 2020Review
- Amyloid beta peptides, locus coeruleus-norepinephrine system and dense core vesicles.Brain research · 2019Review
- Monoamines differentially modulate neuropeptide release from distinct sites within a single neuron pair.PloS one · 2018Article
- Two dipolar α-helices within hormone-encoding regions of proglucagon are sorting signals to the regulated secretory pathway.The Journal of biological chemistry · 2014Article
- Temporal changes in nutritional state affect hypothalamic POMC peptide levels independently of leptin in adult male mice.American journal of physiology. Endocrinology and metabolism · 2014Article
- Central regulation of hypothalamic-pituitary-thyroid axis under physiological and pathophysiological conditions.Endocrine reviews · 2014Review
- ProSAAS-derived peptides are differentially processed and sorted in mouse brain and AtT-20 cells.PloS one · 2014Article
- The functional significance of synaptotagmin diversity in neuroendocrine secretion.Frontiers in endocrinology · 2013Review
- Mechanisms by which the orexigen NPY regulates anorexigenic α-MSH and TRH.American journal of physiology. Endocrinology and metabolism · 2013Article
- β-Endorphin antagonizes the effects of α-MSH on food intake and body weight.Endocrinology · 2012Article
- Release mode of large and small dense-core vesicles specified by different synaptotagmin isoforms in PC12 cells.Molecular biology of the cell · 2011Article
- Regulation of the hypothalamic thyrotropin releasing hormone (TRH) neuron by neuronal and peripheral inputs.Frontiers in neuroendocrinology · 2010Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Prothyrotropin-releasing hormone (pro-TRH) is initially cleaved by the prohormone convertase-1/3 (PC1/3) in the trans-Golgi network generating N- and C-terminal intermediate forms that are then packed into secretory vesicles. However, it is not known whether these peptides are differentially sorted within the secretory pathway. This is of key importance because the processing products of several prohormones fulfill different biological functions. Using AtT20 cells stably transfected with prepro-TRH cDNA, we found that two specific N- and C-terminal peptides were located in different vesicles. Furthermore, the C-terminal pro-TRH-derived peptides were more efficiently released in response to KCl and norepinephrine, a natural secretagogue of TRH. Similar sorting and secretion of N- and C-terminal peptides occurs in vivo. When we blocked the initial proteolytic processing by a mutagenic approach, the differential sorting and secretion of these peptides were prevented. In summary, our data show that pro-TRH-derived peptides are differentially sorted within the secretory pathway and that the initial cleavage in the trans-Golgi network is key to this process. This could be a common mechanism used by neuroendocrine cells to regulate independently the secretion of different bioactive peptides derived from the same gene product.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.