ArticleThe Journal of general virology2008
Properties of non-structural protein 1 of Semliki Forest virus and its interference with virus replication.
Article in The Journal of general virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Japanese encephalitis virus inhibits superinfection of Zika virus in cells by the NS2B protein.Journal of virology · 2024Article
- Superinfection Exclusion in Mosquitoes and Its Potential as an Arbovirus Control Strategy.Viruses · 2020Review
- Fatty Acid Synthase Promotes the Palmitoylation of Chikungunya Virus nsP1.Journal of virology · 2019Article
- Mutational analysis of hepatitis E virus ORF1 "Y-domain": Effects on RNA replication and virion infectivity.World journal of gastroenterology · 2017Article
- Coinfection of tick cell lines has variable effects on replication of intracellular bacterial and viral pathogens.Ticks and tick-borne diseases · 2014Article
- BST-2/tetherin-mediated restriction of chikungunya (CHIKV) VLP budding is counteracted by CHIKV non-structural protein 1 (nsP1).Virology · 2013Article
- Gene silencing in tick cell lines using small interfering or long double-stranded RNA.Experimental & applied acarology · 2013Article
- A systematic analysis of host factors reveals a Med23-interferon-λ regulatory axis against herpes simplex virus type 1 replication.PLoS pathogens · 2013Article
- Noncoding flavivirus RNA displays RNA interference suppressor activity in insect and Mammalian cells.Journal of virology · 2012Article
- Palmitoylation of virus proteins.Biology of the cell · 2012Review
- Antiviral RNA interference responses induced by Semliki Forest virus infection of mosquito cells: characterization, origin, and frequency-dependent functions of virus-derived small interfering RNAs.Journal of virology · 2011Article
- Blocking of melatonin synthesis and MT(1) receptor impairs the activation of Jurkat T cells.Cellular and molecular life sciences : CMLS · 2010Article
- Combined agonist-antagonist genome-wide functional screening identifies broadly active antiviral microRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Novel functions of the alphavirus nonstructural protein nsP3 C-terminal region.Journal of virology · 2010Article
- Cell-to-cell spread of the RNA interference response suppresses Semliki Forest virus (SFV) infection of mosquito cell cultures and cannot be antagonized by SFV.Journal of virology · 2009Article
- Neurons and oligodendrocytes in the mouse brain differ in their ability to replicate Semliki Forest virus.Journal of neurovirology · 2009Article
- Semliki Forest virus strongly reduces mosquito host defence signaling.Insect molecular biology · 2008Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Semliki Forest virus (SFV) non-structural protein 1 (nsP1) is a major component of the virus replicase complex. It has previously been studied in cells infected with virus or using transient or stable expression systems. To extend these studies, tetracycline-inducible stable cell lines expressing SFV nsP1 or its palmitoylation-negative mutant (nsP16D) were constructed. The levels of protein expression and the subcellular localization of nsP1 in induced cells were similar to those in virus-infected cells. The nsP1 expressed by stable, inducible cell lines or by SFV-infected HEK293 T-REx cells was a stable protein with a half-life of approximately 5 h. In contrast to SFV infection, induction of nsP1 expression had no detectable effect on cellular transcription, translation or viability. Induction of expression of nsP1 or nsP16D interfered with multiplication of SFV, typically resulting in a 5-10-fold reduction in virus yields. This reduction was not due to a decrease in the number of infected cells, indicating that nsP1 expression does not block virus entry or initiation of replication. Expression of nsP1 interfered with virus genomic RNA synthesis and delayed accumulation of viral subgenomic RNA translation products. Expression of nsP1 with a mutation in the palmitoylation site reduced synthesis of genomic and subgenomic RNAs and their products of translation, and this effect did not resolve with time. These results are in agreement with data published previously, suggesting a role for nsP1 in genomic RNA synthesis.
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