Evidence map›Paper›PMID 18474218›Full record

ArticleBiochemical and biophysical research communications2008

Pulsatile equibiaxial stretch inhibits thrombin-induced RhoA and NF-kappaB activation.

Jason H Haga, Roland Kaunas, Julie Radeff-Huang, Jessica M Weems, Kristine D Estrada, Shu Chien, Joan Heller Brown, Tammy M Seasholtz

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Jason H HagaDepartment of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-00435, USA. jhaga@bioeng.ucsd.edu
Roland Kaunas
Julie Radeff-Huang
Jessica M Weems
Kristine D Estrada
Shu Chien
Joan Heller Brown
Tammy M Seasholtz
University of California, San Diego · US

Funding

MOLECULAR BASIS OF ENDOTHELIAL MECHANICS AND ADHESIONP01HL043026 · NHLBI · UNIVERSITY OF CALIFORNIA SAN DIEGO · PI SCHMID-SCHOENBEIN, GEERT W. · 1990 to 2005
$8.3M
MOLECULAR BASIS OF ENDOTHELIAL REMODELING BY FLOWR01HL064382 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHIEN, SHU · 1999 to 2009
$6.5M
THROMBIN, GALPHA12, AND RHO AND CELL GROWTHR01GM036927 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BROWN, JOAN HELLER · 1986 to 2012
$3.4M
Mechanical Feedback on Cell Structure and SignalingR01HL080518 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHIEN, SHU · 2006 to 2010
$1.9M
Rho- and GPCR-mediated signaling through novel cellular effectorsR56GM036927 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BROWN, JOAN HELLER · 2008 to 2008
$302k
Cyclic Strain and PDGF in Vascular SMC SignalingF32HL071390 · NHLBI · UNIVERSITY OF CALIFORNIA SAN DIEGO · PI HAGA, JASON H · 2003 to 2003
$46k
NHLBI NIH HHS F32 HL071390NHLBI NIH HHS HL080518NHLBI NIH HHS HL43026NHLBI NIH HHS HL64382NHLBI NIH HHS HL71390NHLBI NIH HHS P01 HL043026NHLBI NIH HHS R01 HL064382NHLBI NIH HHS R01 HL080518NIGMS NIH HHS GM36927NIGMS NIH HHS R01 GM036927NIGMS NIH HHS R56 GM036927
6 · The paper itself

Abstract

This study investigated interactions between the effects of mechanical stretch and thrombin on RhoA activation in rat aortic smooth muscle cells (RASMC). Equibiaxial, pulsatile stretch, or thrombin produced a significant increase in RhoA activation. Surprisingly, in combination, 30 min of stretch inhibited the ability of thrombin to activate RhoA. NO donors and 8-bromo-cGMP significantly inhibited thrombin-induced RhoA activation. Interestingly, the nitric oxide synthase (NOS) inhibitor L-NAME increased basal RhoA activity, suggesting that NOS activity exerts a tonic inhibition on RhoA. Stretching RASMC increases nitrite production, consistent with the idea that NO contributes to the inhibitory effects of stretch. Thrombin stimulates MAP kinase and NF-kappaB pathways through Rho and these responses were blocked by 8-bromo-cGMP or stretch and restored by L-NAME. These data suggest that stretch, acting through NO and cGMP, can prevent the ability of thrombin to stimulate Rho signaling pathways that contribute to pathophysiological proliferative and inflammatory responses.

Indexed as

AnimalsAortaCyclic GMPExtracellular Signal-Regulated MAP KinasesI-kappa B ProteinsMyocytes, Smooth MuscleNF-kappa BNG-Nitroarginine Methyl EsterNitric OxidePhosphorylationRatsRats, Sprague-DawleyrhoA GTP-Binding ProteinSignal TransductionTensile StrengthThrombin8-bromocyclic GMPCyclic GMPExtracellular Signal-Regulated MAP KinasesI kappa B beta proteinI-kappa B ProteinsNF-kappa BNG-Nitroarginine Methyl EsterNitric OxiderhoA GTP-Binding ProteinThrombin

Identifiers

PMID18474218
PMCPMC2587448
OpenAlexW2057081497

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.