ArticleGastroenterology2008
Characterization of fetal and postnatal enteric neuronal cell lines with improvement in intestinal neural function.
Article in Gastroenterology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 74 citations in OpenAlex.
- Western diet induces iron-dependent enteric neurodegeneration via ferroptosis.The Journal of clinical investigation · 2026Article
- Macrophage Immune-Competent Colon Assembloids for Functional Interrogation of Neuroinflammation-Induced Colonic Dysmotility.Gastro hep advances · 2026Article
- Clickable Granular Hydrogel Scaffolds for Delivery of Neural Progenitor Cells to Sites of Spinal Cord Injury.Advanced healthcare materials · 2024Article
- Modulation of Ceramide-Induced Apoptosis in Enteric Neurons by Aryl Hydrocarbon Receptor Signaling: Unveiling a New Pathway beyond ER Stress.International journal of molecular sciences · 2024Article
- Review
- Hypoxia-Inducible Factor 1α Stabilization Restores Epigenetic Control of Nitric Oxide Synthase 1 Expression and Reverses Gastroparesis in Female Diabetic Mice.Gastroenterology · 2023Article
- Aryl hydrocarbon receptor activation affects nitrergic neuronal survival and delays intestinal motility in mice.Toxicological sciences : an official journal of the Society of Toxicology · 2023Article
- SIRT3 activation promotes enteric neurons survival and differentiation.Scientific reports · 2022Article
- Enteric neurosphere cells injected into rectal submucosa might migrate caudorostrally to reconstitute enteric ganglia along the entire length of postnatal colon.Stem cell research & therapy · 2022Article
- Hirschsprung's disease: key microRNAs and target genes.Pediatric research · 2022Article
- Tissue engineering of the gastrointestinal tract: the historic path to translation.Journal of biological engineering · 2022Review
- A functional network of highly pure enteric neurons in a dish.Frontiers in neuroscience · 2022Article
- Phenotypic and functional comparison of rat enteric neural crest-derived cells during fetal and early-postnatal stages.Neural regeneration research · 2021Article
- Modeling Intestinal Stem Cell Function with Organoids.International journal of molecular sciences · 2021Review
- Caspase-11-mediated enteric neuronal pyroptosis underlies Western diet-induced colonic dysmotility.The Journal of clinical investigation · 2020Article
- Bioengineering and regeneration of gastrointestinal tissue: where are we now and what comes next?Expert opinion on biological therapy · 2019Review
- Defining the transcriptomic landscape of the developing enteric nervous system and its cellular environment.BMC genomics · 2017Article
- Western diet induces colonic nitrergic myenteric neuropathy and dysmotility in mice via saturated fatty acid- and lipopolysaccharide-induced TLR4 signalling.The Journal of physiology · 2017Article
- Best practice for passaging murine embryonic enteric neuronal cell line before differentiation.Cytotechnology · 2016Article
- White paper on guidelines concerning enteric nervous system stem cell therapy for enteric neuropathies.Developmental biology · 2016Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
BACKGROUND &
aimsThe isolation and culture of primary enteric neurons is a difficult process and yields a small number of neurons. We developed fetal and postnatal enteric neuronal cell lines using H-2K(b)-tsA58 transgenic mice (immortomice) that have a temperature-sensitive mutation of the SV40 large tumor antigen gene under the control of an interferon gamma-inducible H-2K(b) promoter element.
methodsEnteric neuronal precursors were isolated from the intestines of E13-mouse fetuses and second day postnatal mice using magnetic immunoselection with a p75NTR antibody. The cells were maintained at the permissive temperature, 33 degrees C, and interferon-gamma for 24 or 48 hours, and then transferred to 39 degrees C in the presence of glial cell line-derived neurotrophic factor for 7 days for further differentiation. Neuronal markers were assessed by reverse-transcription polymerase chain reaction, Western blot, and immunocytochemistry. Neuronal function was assessed by transplanting these cells into the colons of Piebald or nNOS(-/-) mice.
resultsExpression analysis of cells showed the presence of neuronal markers peripherin, PGP9.5, HuD, tau, synaptic marker synaptophysin, characteristic receptors of enteric neurons, Ret, and 5-hydroxytryptamine-receptor subtypes at 33 degrees C and 39 degrees C. Nestin, S-100beta, and alpha-smooth muscle actin were expressed minimally at 39 degrees C. Glial cell line-derived neurotrophic factor resulted in increased phosphorylation of Akt in these cells, similar to primary enteric neurons. Transplantation of cells into the piebald or nNOS(-/-) mice colon improved colonic motility.
conclusionsWe have developed novel enteric neuronal cell lines that have neuronal characteristics similar to primary enteric neurons. These cells can help us in understanding newer therapeutic options for Hirschsprung's disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.