ArticleParasites & vectors2008
Canine leishmaniasis in Southern Italy: a role for nitric oxide released from activated macrophages in asymptomatic infection?
Article in Parasites & vectors, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Isolation ofOne health (Amsterdam, Netherlands) · 2026Article
- Leishmania infantum in red foxes (Vulpes vulpes): from clinical findings to cytokine expression.Parasites & vectors · 2026Article
- Expression of key cytokines in dog macrophages infected by Leishmania tarentolae opening new avenues for the protection against Leishmania infantum.Scientific reports · 2024Article
- Interaction between Wolbachia pipientis and Leishmania infantum in heartworm infected dogs.Parasites & vectors · 2023Article
- MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis.Frontiers in genetics · 2023Article
- Humoral and Cellular Immune Response in Asymptomatic Dogs with Visceral Leishmaniasis: A Review.Vaccines · 2022Review
- Epidemiologic, Clinical and Immunological Consequences of Co-Infections during Canine Leishmaniosis.Animals : an open access journal from MDPI · 2021Review
- Clinical Significance of ROMs, OXY, SHp and HMGB-1 in Canine Leishmaniosis.Animals : an open access journal from MDPI · 2021Article
- Biomarkers Associated WithFrontiers in cellular and infection microbiology · 2018Review
- Canine β-defensin-1 (CBD1) gene as a possible marker for Leishmania infantum infection in dogs.Parasites & vectors · 2017Article
- Insights on adaptive and innate immunity in canine leishmaniosis.Parasitology · 2017Article
- Programmed death 1-mediated T cell exhaustion during visceral leishmaniasis impairs phagocyte function.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Canine visceral leishmaniasis: relationships between oxidative stress, liver and kidney variables, trace elements, and clinical status.Parasitology research · 2012Article
- Animal models for the study of leishmaniasis immunology.Revista do Instituto de Medicina Tropical de Sao PauloReview
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelled
backgroundHuman and canine leishmaniasis (CanL) by Leishmania infantum is endemic in Italy, with a high percentage of infected asymptomatic animals. However, the immune response mechanisms underlying the clinical presentation of CanL have not been fully investigated. Among leishmanicidal molecules produced by activated macrophages, nitric oxide (NO) produced by an inducible NO synthase seems to play an important protective role, but no conclusive data are available. Therefore, NO released by cultured macrophages from dogs with natural Leishmania infection living in an endemic area for CanL was evaluated.
methodsOn the basis of one year's clinical and laboratory follow-up, 22 dogs infected by Leishmania infantum were identified and grouped as: asymptomatic dogs (n = 13) and dogs with symptoms of leishmaniasis (n = 9). Each animal was bled twice at 4-month intervals and macrophage and lymphocyte cultures were obtained from peripheral blood mononuclear cells. Supernatants of L. infantum-infected macrophage cultures, with or without addition of autologous lymphocytes, were assayed for NO production by Griess reaction for nitrites.
resultsIn the first months of the infection the levels of NO in supernatants of Leishmania-infected macrophages were higher in symptomatic than in asymptomatic dogs, but they were significantly increased in the latter group eight months after the diagnosis of infection. Furthermore, NO release significantly decreased in the presence of autologous lymphocytes in both groups of animals.
conclusionThese results suggest that NO may be involved in the long-term protection of dogs against natural Leishmania infection and in the clinical presentation of canine leishmaniasis in the Mediterranean area.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.