Evidence map›Paper›PMID 18469824›Full record

ArticleNature methods2008

Next-generation high-density self-assembling functional protein arrays.

Niroshan Ramachandran, Jacob V Raphael, Eugenie Hainsworth, Gokhan Demirkan, Manuel G Fuentes, Andreas Rolfs, Yanhui Hu, Joshua LaBaer

Abstract read
In one paragraph

Article in Nature methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 138 papers.

0numbers the graph read from it
0cells of the map it votes in
138citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

138 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. In Vitro GT-array (Bio-protocol · 2024
    Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Review
  12. Comparative Microbiomics Analysis of Antimicrobial Antibody Response between Patients with Lung Cancer and Control Subjects with Benign Pulmonary Nodules.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2023
    Article
  13. Review
  14. Article
  15. Article
  16. Article
  17. Identification of anti-Epstein-Barr virus (EBV) antibody signature in EBV-associated gastric carcinoma.Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association · 2021
    Article
  18. Article
  19. Analysis of Protein-Protein Interactions by Protein Microarrays.Methods in molecular biology (Clifton, N.J.) · 2021
    Article
  20. Review

78 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Niroshan RamachandranHarvard Institute of Proteomics, Harvard Medical School, 320 Charles Street, Cambridge, Massachusetts 02141, USA.
Jacob V Raphael
Eugenie Hainsworth
Gokhan Demirkan
Manuel G Fuentes
Andreas Rolfs
Yanhui Hu
Joshua LaBaer

Funding

Biomarker Detection Using NAPPA Tumor Antigen ArraysU01CA117374 · NCI · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI ANDERSON, KAREN SUE, LABAER, JOSHUA · 2005 to 2015
$6.2M
NCI NIH HHS 5U01CA117374-02NCI NIH HHS U01 CA117374PHS HHS HHSN2332200400053C
6 · The paper itself

Abstract

We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.

Indexed as

AlbuminsAnimalsCattleCell-Free SystemCloning, MolecularDNA, ComplementaryFluorescent DyesGene Expression ProfilingGlutathione TransferaseHumansOligonucleotide Array Sequence AnalysisPeptide LibraryProtein Array AnalysisProteomicsAlbuminsDNA, ComplementaryFluorescent DyesGlutathione TransferasePeptide Library

Identifiers

PMID18469824
PMCPMC3070491

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.