ArticleInvestigative ophthalmology & visual science2008
Reprogramming progeny cells of embryonic RPE to produce photoreceptors: development of advanced photoreceptor traits under the induction of neuroD.
Article in Investigative ophthalmology & visual science, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Photoreceptor-like cells in transgenic mouse eye.Investigative ophthalmology & visual science · 2013Article
- Amniotic fluid promotes the appearance of neural retinal progenitors and neurons in human RPE cell cultures.Molecular vision · 2013Article
- Photoreceptor-like cells from reprogramming cultured mammalian RPE cells.Molecular vision · 2013Article
- Chick retinal pigment epithelium transdifferentiation assay for proneural activities.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Generating retinal neurons by reprogramming retinal pigment epithelial cells.Expert opinion on biological therapy · 2010Review
- Neurogenin1 effectively reprograms cultured chick retinal pigment epithelial cells to differentiate toward photoreceptors.The Journal of comparative neurology · 2010Article
- Using neurogenin to reprogram chick RPE to produce photoreceptor-like neurons.Investigative ophthalmology & visual science · 2010Article
- Selective survival rescue in 15-lipoxygenase-1-deficient retinal pigment epithelial cells by the novel docosahexaenoic acid-derived mediator, neuroprotectin D1.The Journal of biological chemistry · 2009Article
- Reprogramming chick RPE progeny cells to differentiate towards retinal neurons by ash1.Molecular vision · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeIn examining the prospect of producing functional photoreceptors by reprogramming the differentiation of RPE progeny cells, this study was conducted to investigate whether reprogrammed cells can develop highly specialized ultrastructural and physiological traits that characterize retinal photoreceptors.
methodsCultured chick RPE cells were reprogrammed to differentiate along the photoreceptor pathway by ectopic expression of neuroD. Cellular ultrastructure was examined with electron microscopy. Cellular physiology was studied by monitoring cellular free calcium (Ca(2+)) levels in dark-adapted cells in response to light and in light-bleached cells in response to 9-cis-retinal.
resultsReprogrammed cells were found to localize red opsin protein appropriately to the apex. These cells developed inner segments rich in mitochondria, and while in culture, some formed rudimentary outer segments, analogous to those of developing photoreceptors in the retina. In response to light, reprogrammed cells reduced their Ca(2+) levels, as observed with developing retinal photoreceptors in culture. Further, on exposure to 9-cis-retinal, the light-bleached, reprogrammed cells increased their Ca(2+) levels, reminiscent of visual cycle recovery.
conclusionsThese results indicate the potential of reprogrammed cells to develop advanced ultrastructural and physiological traits of photoreceptors and point to reprogramming progeny cells of embryonic RPE as a possible alternative in producing developing photoreceptors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.