ArticleJournal of bacteriology2008
Characterization of Clostridium perfringens spores that lack SpoVA proteins and dipicolinic acid.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 98 citations in OpenAlex.
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- Isolation of a VirulentCurrent issues in molecular biology · 2024Article
- The small acid-soluble proteins of spore-forming organisms: similarities and differences in function.Anaerobe · 2024Review
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- Characterization of Putative Sporulation and Germination Genes inMicroorganisms · 2022Article
- Occurrence of genes encoding spore germination inMicrobial genomics · 2022Article
- Sporulation in solventogenic and acetogenic clostridia.Applied microbiology and biotechnology · 2021Review
- Biofilm and Spore Formation ofAntibiotics (Basel, Switzerland) · 2021Article
- Sporulation and Germination in Clostridial Pathogens.Microbiology spectrum · 2019Review
- Updates to Clostridium difficile Spore Germination.Journal of bacteriology · 2018Review
- Revisiting the Role of Csp Family Proteins in Regulating Clostridium difficile Spore Germination.Journal of bacteriology · 2017Article
- Intestinal calcium and bile salts facilitate germination of Clostridium difficile spores.PLoS pathogens · 2017Article
- Genetic Characterization of the Exceptionally High Heat Resistance of the Non-toxic SurrogateFrontiers in microbiology · 2017Article
- Effects of High-Pressure Treatment on Spores of Clostridium Species.Applied and environmental microbiology · 2016Article
- Characterization of Clostridium difficile Spores Lacking Either SpoVAC or Dipicolinic Acid Synthetase.Journal of bacteriology · 2016Article
- Clostridium perfringens Sporulation and Sporulation-Associated Toxin Production.Microbiology spectrum · 2016Review
- Regulation of Clostridium difficile spore germination by the CspA pseudoprotease domain.Biochimie · 2016Article
- Diversity of the Germination Apparatus inFrontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spores of Clostridium perfringens possess high heat resistance, and when these spores germinate and return to active growth, they can cause gastrointestinal disease. Work with Bacillus subtilis has shown that the spore's dipicolinic acid (DPA) level can markedly influence both spore germination and resistance and that the proteins encoded by the spoVA operon are essential for DPA uptake by the developing spore during sporulation. We now find that proteins encoded by the spoVA operon are also essential for the uptake of Ca(2+) and DPA into the developing spore during C. perfringens sporulation. Spores of a spoVA mutant had little, if any, Ca(2+) and DPA, and their core water content was approximately twofold higher than that of wild-type spores. These DPA-less spores did not germinate spontaneously, as DPA-less B. subtilis spores do. Indeed, wild-type and spoVA C. perfringens spores germinated similarly with a mixture of l-asparagine and KCl (AK), KCl alone, or a 1:1 chelate of Ca(2+) and DPA (Ca-DPA). However, the viability of C. perfringens spoVA spores was 20-fold lower than the viability of wild-type spores. Decoated wild-type and spoVA spores exhibited little, if any, germination with AK, KCl, or exogenous Ca-DPA, and their colony-forming efficiency was 10(3)- to 10(4)-fold lower than that of intact spores. However, lysozyme treatment rescued these decoated spores. Although the levels of DNA-protective alpha/beta-type, small, acid-soluble spore proteins in spoVA spores were similar to those in wild-type spores, spoVA spores exhibited markedly lower resistance to moist heat, formaldehyde, HCl, hydrogen peroxide, nitrous acid, and UV radiation than wild-type spores did. In sum, these results suggest the following. (i) SpoVA proteins are essential for Ca-DPA uptake by developing spores during C. perfringens sporulation. (ii) SpoVA proteins and Ca-DPA release are not required for C. perfringens spore germination. (iii) A low spore core water content is essential for full resistance of C. perfringens spores to moist heat, UV radiation, and chemicals.
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