ArticleBiophysical journal2008
Kinetics, statistics, and energetics of lipid membrane electroporation studied by molecular dynamics simulations.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
107 citing papers in PubMed.
- Biophysical principles of pulsed field ablation.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2026Review
- Investigating the Role of Water Molecules in TRPV4 Ion Channels Under Intense Electric Fields Using Molecular Dynamics Simulations.Bioelectricity · 2026Article
- From Membrane Composition to Antimicrobial Strategies: Experimental and Computational Approaches to AMP Design and Selectivity.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Mathematical Modeling and Computational Approaches for Pulsed Electric Field Processing in Food Preservation: A Comprehensive Review.Foods (Basel, Switzerland) · 2026Review
- A Methodology for Deciphering the Transmembrane Resistance Variability of Supported Lipid Bilayers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Distinct Structural Responses of Lipid Bilayers to Horizontal and Vertical Electric Fields.The journal of physical chemistry letters · 2025Article
- Atomistic modeling of lysophospholipids from the Campylobacter jejuni lipidome.Biophysical journal · 2025Article
- Optimizing electroporation via pulse modulation: a molecular dynamics study.European biophysics journal : EBJ · 2025Article
- Effects of Interphase and Interpulse Delays on Tissue Impedance and Pulsed Field Ablation.Annals of biomedical engineering · 2025Article
- Pulsed field ablation in medicine: irreversible electroporation and electropermeabilization theory and applications.Radiology and oncology · 2025Review
- How pore formation in complex biological membranes is governed by lipid composition, mechanics, and lateral sorting.PNAS nexus · 2025Article
- Power-Driven Electroporation Is Predictive of Treatment Outcomes in a Conductivity-Independent Manner.BME frontiers · 2025Article
- Article
- Cell-inspired, massive electromodulation of friction via transmembrane fields across lipid bilayers.Nature materials · 2024Article
- Numerical assessments of geometry, proximity and multi-electrode effects on electroporation in mitochondria and the endoplasmic reticulum to nanosecond electric pulses.Scientific reports · 2024Article
- Effects of Normal and Lateral Electric Fields on Membrane Mechanical Properties.The journal of physical chemistry. B · 2024Article
- Probability and kinetics of rupture and electrofusion in giant unilamellar vesicles under various frequencies of direct current pulses.PloS one · 2024Article
- Article
- Electric-field frictional effects in confined zwitterionic molecules.Physical chemistry chemical physics : PCCP · 2023Article
- High curvature promotes fusion of lipid membranes: Predictions from continuum elastic theory.Biophysical journal · 2023Article
47 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane electroporation is the method to directly transfer bioactive substances such as drugs and genes into living cells, as well as preceding electrofusion. Although much information on the microscopic mechanism has been obtained both from experiment and simulation, the existence and nature of possible intermediates is still unclear. To elucidate intermediates of electropore formation by direct comparison with measured prepore formation kinetics, we have carried out 49 atomistic electroporation simulations on a palmitoyl-oleoyl-phosphatidylcholine bilayer for electric field strengths between 0.04 and 0.7 V/nm. A statistical theory is developed to facilitate direct comparison of experimental (macroscopic) prepore formation kinetics with the (single event) preporation times derived from the simulations, which also allows us to extract an effective number of lipids involved in each pore formation event. A linear dependency of the activation energy for prepore formation on the applied field is seen, with quantitative agreement between experiment and simulation. The distribution of preporation times suggests a four-state pore formation model. The model involves a first intermediate characterized by a differential tilt of the polar lipid headgroups on both leaflets, and a second intermediate (prepore), where a polar chain across the bilayer is formed by 3-4 lipid headgroups and several water molecules, thereby providing a microscopic explanation for the polarizable volume derived previously from the measured kinetics. An average pore radius of 0.47 +/- 0.15 nm is seen, in favorable agreement with conductance measurements and electrooptical experiments of lipid vesicles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.