ArticleJournal of structural biology2008
Detection and imaging of non-contractile inclusions and sarcomeric anomalies in skeletal muscle by second harmonic generation combined with two-photon excited fluorescence.
Article in Journal of structural biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed.
- Neuromechanical basis of region-specific differences and their implications for sport performance and injury prevention: a narrative review.European journal of applied physiology · 2025Review
- Spatial polarimetric second harmonic generation evaluation of collagen in a hypophosphatasia mouse model.Biomedical optics express · 2024Article
- Failure of Autophagy in Pompe Disease.Biomolecules · 2024Review
- How to estimate the sarcomere size based on oblique sections of skeletal muscle.Journal of anatomy · 2023Article
- Second harmonic generation microscopy: a powerful tool for bio-imaging.Biophysical reviews · 2023Review
- Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization.Lasers in medical science · 2021Article
- Enzyme Replacement Therapy Can Reverse Pathogenic Cascade in Pompe Disease.Molecular therapy. Methods & clinical development · 2020Article
- Second harmonic generation characterization of collagen in whole bone.Biomedical optics express · 2020Article
- The unified myofibrillar matrix for force generation in muscle.Nature communications · 2020Article
- Advancements in AAV-mediated Gene Therapy for Pompe Disease.Journal of neuromuscular diseases · 2020Review
- Pros and cons of different ways to address dysfunctional autophagy in Pompe disease.Annals of translational medicine · 2019Review
- Improved efficacy of a next-generation ERT in murine Pompe disease.JCI insight · 2019Article
- Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach.Light, science & applications · 2018Article
- In Vivo Sarcomere Length Measurement in Whole Muscles during Passive Stretch and Twitch Contractions.Biophysical journal · 2017Article
- Article
- Clearing skeletal muscle with CLARITY for light microscopy imaging.Cell biology international · 2016Article
- Functional second harmonic generation microscopy probes molecular dynamics with high temporal resolution.Biomedical optics express · 2016Article
- In vivo Sarcomere Lengths and Sarcomere Elongations Are Not Uniform across an Intact Muscle.Frontiers in physiology · 2016Article
- Article
- Myofibrillar misalignment correlated to triad disappearance of mdx mouse gastrocnemius muscle probed by SHG microscopy.Biomedical optics express · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
The large size of the multinucleated muscle fibers of skeletal muscle makes their examination for structural and pathological defects a challenge. Sections and single fibers are accessible to antibodies and other markers but imaging of such samples does not provide a three-dimensional view of the muscle. Regrettably, bundles of fibers cannot be stained or imaged easily. Two-photon microscopy techniques overcome these obstacles. Second harmonic generation (SHG) by myosin filaments and two-photon excited fluorescence (2PEF) of mitochondrial and lysosomal components provides detailed structural information on unstained tissue. Furthermore, the infrared exciting light can penetrate several layers of muscle fibers and the minimal processing is particularly valuable for fragile biopsies. Here we demonstrate the usefulness of SHG, combined with 2PEF, to reveal enlarged lysosomes and accumulations of non-contractile material in muscles from the mouse model for the lysosomal storage disorder Pompe disease (PD), and in biopsies from adult and infant PD patients. SHG and 2PEF also detect sarcomeric defects that may presage the loss of myofibrils in atrophying muscle and signify loss of elasticity. The combination of SHG and 2PEF should be useful in the analysis and diagnosis of a wide range of skeletal muscle pathologies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.