ArticleNeuroscience letters2008
Cell-cell interaction modulates neuroectodermal specification of embryonic stem cells.
Article in Neuroscience letters, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 62 citations in OpenAlex.
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- Engineering Biomimetic Trogocytosis with Farnesylated Chemically Self-Assembled Nanorings.Biomacromolecules · 2022Article
- Manipulating Cell Fates with Protein Conjugates.Bioconjugate chemistry · 2022Review
- Cell-Cell Contact Mediates Gene Expression and Fate Choice of Human Neural Stem/Progenitor Cells.Cells · 2022Article
- Connexins in the development and physiology of stem cells.Tissue barriers · 2021Review
- Novel Approaches Used to Examine and Control Neurogenesis in Parkinson's Disease.International journal of molecular sciences · 2021Review
- Fluorescence-based actin turnover dynamics of stem cells as a profiling method for stem cell functional evolution, heterogeneity and phenotypic lineage parsing.Methods (San Diego, Calif.) · 2021Article
- A Microfluidic Probe Integrated Device for Spatiotemporal 3D Chemical Stimulation in Cells.Micromachines · 2020Article
- Microfluidic device performing on flow study of serial cell-cell interactions of two cell populations.RSC advances · 2019Article
- Stem cell colony interspacing effect on differentiation to neural cells.Journal of tissue engineering and regenerative medicine · 2018Article
- Increased stem cells delivered using a silk gel/scaffold complex for enhanced bone regeneration.Scientific reports · 2017Article
- Neurotrauma: The Crosstalk between Neurotrophins and Inflammation in the Acutely Injured Brain.International journal of molecular sciences · 2017Review
- A Gap Junction Protein, Inx2, Modulates Calcium Flux to Specify Border Cell Fate during Drosophila oogenesis.PLoS genetics · 2017Article
- Neuroinflammation in animal models of traumatic brain injury.Journal of neuroscience methods · 2016Review
- The niche in single-cell technologies.Immunology and cell biology · 2016Review
- Methylene blue exerts a neuroprotective effect against traumatic brain injury by promoting autophagy and inhibiting microglial activation.Molecular medicine reports · 2016Article
- Neural differentiation from embryonic stem cells in vitro: An overview of the signaling pathways.World journal of stem cells · 2015Review
- Connexin43 is required for the maintenance of multipotency in skin-derived stem cells.Stem cells and development · 2014Article
- Cross-regulation of Connexin43 and β-catenin influences differentiation of human neural progenitor cells.Cell death & disease · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
The controlled differentiation of embryonic stem (ES) cells is of utmost interest to their clinical, biotechnological, and basic science use. Many investigators have combinatorially assessed the role of specific soluble factors and extracellular matrices in guiding ES cell fate, yet the interaction between neighboring cells in these heterogeneous cultures has been poorly defined due to a lack of conventional tools to specifically uncouple these variables. Herein, we explored the role of cell-cell interactions during neuroectodermal specification of ES cells using a microfabricated cell pair array. We tracked differentiation events in situ, using an ES cell line expressing green fluorescent protein (GFP) under the regulation of the Sox1 gene promoter, an early marker of neuroectodermal germ cell commitment in the adult forebrain. We observed that a previously specified Sox1-GFP+ cell could induce the specification of an undifferentiated ES cell. This induction was modulated by the two cells being in contact and was dependent on the age of previously specified cell prior to coculture. A screen of candidate cell adhesion molecules revealed that the expression of connexin (Cx)-43 correlated with the age-dependent effect of cell contact in cell pair experiments. ES cells deficient in Cx-43 showed aberrant neuroectodermal specification and lineage commitment, highlighting the importance of gap junctional signaling in the development of this germ layer. Moreover, this study demonstrates the integration of microscale culture techniques to explore the biology of ES cells and gain insight into relevant developmental processes otherwise undefined due to bulk culture methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.