ArticleMolecular and cellular biology1991
Alpha B crystallin accumulation is a specific response to Ha-ras and v-mos oncogene expression in mouse NIH 3T3 fibroblasts.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 93 citations in OpenAlex.
- Endoplasmic Reticulum Stress of Gut Enterocyte and Intestinal Diseases.Frontiers in molecular biosciences · 2022Review
- Regulation of αA- and αB-crystallins via phosphorylation in cellular homeostasis.Cellular and molecular life sciences : CMLS · 2015Review
- Possible predictors of histopathological response to neoadjuvant chemoradiotherapy for rectal cancer.Journal of cancer research and clinical oncology · 2012Article
- Different experimental approaches in modelling cataractogenesis: An overview of selenite-induced nuclear cataract in rats.Interdisciplinary toxicology · 2010Article
- Article
- Differences in properties between human alphaA- and alphaB-crystallin proteins expressed in Escherichia coli cells in response to cold and extreme pH.The Biochemical journal · 2003Article
- Evaluation of hydrophobicity versus chaperonelike activity of bovine alphaA- and alphaB-crystallin.Journal of protein chemistry · 2002Article
- Modulation of the chaperone-like activity of bovine alpha-crystallin.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Thermal response in murine L929 cells lacking alpha B-crystallin expression and alpha B-crystallin expressing L929 transfectants.Molecular and cellular biochemistry · 1996Article
- Kinetic changes of alpha B crystallin expression in neoplastic cells and syngeneic rat fibroblasts at various subculture stages.Molecular and cellular biochemistry · 1995Article
- Structure and alternate tissue-preferred transcription initiation of the mouse alpha B-crystallin/small heat shock protein gene.Nucleic acids research · 1994Article
- Growth factor-mediated induction of the delayed early gene T1 depends on a 12-O-tetradecanoylphorbol 13-acetate-responsive element located 3.6 kb upstream of the transcription initiation site.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Alpha-crystallin/small heat shock protein has autokinase activity.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Identification of a lens-specific regulatory region (LSR) of the murine alpha B-crystallin gene.Nucleic acids research · 1994Article
- The murine alpha B-crystallin/small heat shock protein enhancer: identification of alpha BE-1, alpha BE-2, alpha BE-3, and MRF control elements.Molecular and cellular biology · 1993Article
- Alpha B-crystallin expression in mouse NIH 3T3 fibroblasts: glucocorticoid responsiveness and involvement in thermal protection.Molecular and cellular biology · 1993Article
- Expression of the murine small heat shock proteins hsp 25 and alpha B crystallin in the absence of stress.The Journal of cell biology · 1993Article
- The twelfth Frederick H. Verhoeff Lecture: gene sharing in the visual system.Transactions of the American Ophthalmological Society · 1993Review
- Alpha-crystallin can function as a molecular chaperone.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- A superfamily in the mammalian eye lens: the beta/gamma-crystallins.Molecular biology reports · 1992Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conditional expression of the v-mos and Ha-ras(EJ) oncogenes in NIH 3T3 cells leads to the accumulation of a 23-kDa protein (p23) (R. Klemenz, S. Hoffmann, R. Jaggi, and A.-K. Werenskiold, Oncogene 4:799-803, 1989). We purified p23 to homogeneity and determined part of the amino acid sequence. The obtained sequence is identical with that of the eye lens protein alpha B crystallin. Northern (RNA) blot and Western immunoblot experiments were performed to demonstrate that alpha B crystallin mRNA and protein do indeed accumulate as a consequence of v-mos and Ha-ras oncogene expression. Comparison of cDNA clones obtained from the mRNA of eye lenses and of oncogene-expressing fibroblasts revealed identity between them. The major transcription initiation site of the alpha B crystallin gene in our experimental system was shown by primer extension experiments to be identical with the one used in eye epithelial cells. In addition, we identified a second minor initiation site 49 nucleotides further upstream. Serum growth factors did not stimulate alpha B crystallin expression in growth-arrested cells.
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