ArticleMolecular and cellular biology1991
Two types of TATA elements for the CYC1 gene of the yeast Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 69 citations in OpenAlex.
- Article
- A Synthetic Hybrid Promoter for Xylose-Regulated Control of Gene Expression in Saccharomyces Yeasts.Molecular biotechnology · 2017Article
- Engineering prokaryotic transcriptional activators as metabolite biosensors in yeast.Nature chemical biology · 2016Article
- Sequence-directed nucleosome-depletion is sufficient to activate transcription from a yeast core promoter in vivo.Biochemical and biophysical research communications · 2016Article
- Article
- In silico design and in vivo implementation of yeast gene Boolean gates.Journal of biological engineering · 2014Article
- Tunable and multifunctional eukaryotic transcription factors based on CRISPR/Cas.ACS synthetic biology · 2013Article
- Re-engineering multicloning sites for function and convenience.Nucleic acids research · 2011Article
- Minimal components of the RNA polymerase II transcription apparatus determine the consensus TATA box.Nucleic acids research · 2008Article
- Enhanced mitochondrial degradation of yeast cytochrome c with amphipathic structures.Current genetics · 2005Article
- Distinct transcriptional responses of RNA polymerases I, II and III to aptamers that bind TBP.Nucleic acids research · 2005Article
- The role of nuclear cap binding protein Cbc1p of yeast in mRNA termination and degradation.Molecular and cellular biology · 2000Article
- Article
- Molecular genetics of the RNA polymerase II general transcriptional machinery.Microbiology and molecular biology reviews : MMBR · 1998Review
- SWI-SNF complex participation in transcriptional activation at a step subsequent to activator binding.Molecular and cellular biology · 1998Article
- Degradation of CYC1 mRNA in the yeast Saccharomyces cerevisiae does not require translation.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Functional interaction between TFIIB and the Rpb9 (Ssu73) subunit of RNA polymerase II in Saccharomyces cerevisiae.Nucleic acids research · 1996Article
- Signals sufficient for 3'-end formation of yeast mRNA.Molecular and cellular biology · 1996Article
- Mechanism of differential utilization of the his3 TR and TC TATA elements.Molecular and cellular biology · 1995Article
- A bipartite operator interacts with a heat shock element to mediate early meiotic induction of Saccharomyces cerevisiae HSP82.Molecular and cellular biology · 1995Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Functional TATA elements in the 5' untranslated region of the CYC1 gene in the yeast Saccharomyces cerevisiae have been defined by transcriptional analysis of site-directed mutations. Five sites previously suggested to contain functional TATA elements were altered individually and in all possible combinations. The results indicated that only two elements are required for transcription at the normal level and the normal start sites. The two functional TATA elements are located at sites -178 and -123, where the A of the ATG start codon is assigned nucleotide position +1. They direct initiation within windows encompassing -70 to -46 and -46 to -28, respectively. Only when both of the upstream TATA sites were rendered nonfunctional were the third and fourth downstream TATA-like sequences activated, as indicated by the presence of low levels of transcription starting at -28. The two upstream functional TATA elements differed in sequence. The sequence of the most 5' one at site 1, denoted beta-type, was ATATATATAT, whereas that of the second one at site 2, denoted alpha-type, was TATATAAAA. The following rearrangements of the beta-type and alpha-type elements at two sites (1 and 2) were examined: site1 beta-site2 alpha; site 1 alpha-site 2 beta; site1 alpha-site2 alpha; and site1 beta-site2 beta. When different types were at different sites (site1 beta-site2 alpha and site1 alpha-site2 beta), both were used equally. In contrast, when the same type was present at both sites (site1 alpha-site2 alpha and site1 beta-site2 beta), only the upstream element was used. We suggest that the two TATA elements are recognized by different factors of the transcription apparatus.
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