ArticleThe EMBO journal1991
Identification of the site of interaction of the dihydropyridine channel blockers nitrendipine and azidopine with the calcium-channel alpha 1 subunit.
Article in The EMBO journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- The Expanding Role of Pyridine and Dihydropyridine Scaffolds in Drug Design.Drug design, development and therapy · 2021Review
- A Ni(2+)-sensitive component of the ERG b-wave from the isolated bovine retina is related to E-type voltage-gated Ca(2+) channels.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2005Article
- Molecular basis of drug interaction with L-type Ca2+ channels.Journal of bioenergetics and biomembranes · 1998Review
- Voltage-dependent binding and calcium channel current inhibition by an anti-alpha 1D subunit antibody in rat dorsal root ganglion neurones and guinea-pig myocytes.The Journal of physiology · 1997Article
- Dihydropyridine action on voltage-dependent potassium channels expressed in Xenopus oocytes.The Journal of general physiology · 1997Article
- Effects of calciseptine on unitary barium channel currents in guinea-pig portal vein.Pflugers Archiv : European journal of physiology · 1996Article
- The IVS6 segment of the L-type calcium channel is critical for the action of dihydropyridines and phenylalkylamines.The EMBO journal · 1996Article
- Structural model of a synthetic Ca2+ channel with bound Ca2+ ions and dihydropyridine ligand.Biophysical journal · 1996Article
- Two stable cell lines for screening of calcium channel blockers.Naunyn-Schmiedeberg's archives of pharmacology · 1995Article
- Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes.Naunyn-Schmiedeberg's archives of pharmacology · 1995Article
- Calcium current reactivation after flash photolysis of nifedipine in skeletal muscle fibres of the frog.The Journal of physiology · 1995Article
- Characteristics of [125I]omega-conotoxin labeling using bifunctional cross linker DSP in crude membranes from chick brain.Neurochemical research · 1995Article
- Design of a functional calcium channel protein: inferences about an ion channel-forming motif derived from the primary structure of voltage-gated calcium channels.Protein science : a publication of the Protein Society · 1993Article
- The benzazepine/benzothiazepine binding domain of the cardiac L-type Ca2+ channel is accessible only from the extracellular side.Pflugers Archiv : European journal of physiology · 1993Article
- The molecular mode of action of the Ca agonist (-) BAY K 8644 on the cardiac Ca channel.Pflugers Archiv : European journal of physiology · 1993Article
- Evidence for an external location of the dihydropyridine agonist receptor site on smooth muscle and skeletal muscle calcium channels.British journal of pharmacology · 1993Article
- The action of amyotrophic lateral sclerosis immunoglobulins on mammalian single skeletal muscle Ca2+ channels.The Journal of physiology · 1993Article
- Ion channels.Diabetologia · 1992Review
- Modulation of calcium current gating in frog skeletal muscle by conditioning depolarization.The Journal of physiology · 1992Article
- Potentiation of cardiodepressive action among calcium antagonists from different classes: evidence for a mechanism at the single calcium channel level.Naunyn-Schmiedeberg's archives of pharmacology · 1992Article
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5 authors.
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Abstract
The dihydropyridine binding site of the rabbit skeletal muscle calcium channel alpha 1 subunit was identified using tritiated azidopine and nitrendipine as ligands. The purified receptor complex was incubated either with azidopine or nitrenidpine at an alpha 1 subunit to ligand ratio of 1:1. The samples were then irradiated by a 200 W UV lamp. The ligands were only incorporated into the alpha 1 subunit, which was isolated by size exclusion chromatography and digested either by trypsin (azidopine) or endoproteinase Asp-N (nitrendipine). Each digest contained two radioactive peptides, which were isolated and sequenced. The azidopine peptides were identical with amino acids 13-18 (minor peak) and 1428-1437 (major peak) of the primary sequence of the skeletal muscle alpha 1 subunit. The nitrendipine peptides were identical with amino acids 1390-1399 (major peak) and 1410-1420 (minor peak). The sequence from amino acids 1390 to 1437 is identical in the alpha 1 subunits of skeletal, cardiac and smooth muscle and follows directly repeat IVS6. These results indicate that dihydropyridines bind to an area that is located at the putative cytosolic domain of the calcium channel.
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