ArticleBiochemistry2008
Structure and function of the sterol carrier protein-2 N-terminal presequence.
Article in Biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Sterol Carrier Protein X (SCP-x) Regulates Cholesterol Transport in the Migratory LocustBiology · 2026Article
- Sterol carrier protein 2: A promising target in the pathogenesis of atherosclerosis.Genes & diseases · 2023Review
- Effect of liver fatty acid binding protein (L-FABP) gene ablation on lipid metabolism in high glucose diet (HGD) pair-fed mice.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2019Article
- Ablating both Fabp1 and Scp2/Scpx (TKO) induces hepatic phospholipid and cholesterol accumulation in high fat-fed mice.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2018Article
- Sex-dependent impact of Scp-2/Scp-x gene ablation on hepatic phytol metabolism.Archives of biochemistry and biophysics · 2017Article
- Impact ofJournal of lipid research · 2017Article
- Effect of Fabp1/Scp-2/Scp-x Ablation on Whole Body and Hepatic Phenotype of Phytol-Fed Male Mice.Lipids · 2017Article
- Impact of dietary phytol on lipid metabolism in SCP2/SCPX/L-FABP null mice.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Article
- Female Mice are Resistant to Fabp1 Gene Ablation-Induced Alterations in Brain Endocannabinoid Levels.Lipids · 2016Article
- FABP-1 gene ablation impacts brain endocannabinoid system in male mice.Journal of neurochemistry · 2016Article
- LysGH15 kills Staphylococcus aureus without being affected by the humoral immune response or inducing inflammation.Scientific reports · 2016Article
- Relative contributions of L-FABP, SCP-2/SCP-x, or both to hepatic biliary phenotype of female mice.Archives of biochemistry and biophysics · 2015Article
- Impact of SCP-2/SCP-x gene ablation and dietary cholesterol on hepatic lipid accumulation.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Loss of L-FABP, SCP-2/SCP-x, or both induces hepatic lipid accumulation in female mice.Archives of biochemistry and biophysics · 2015Article
- Synthesis, purification and crystallographic studies of the C-terminal sterol carrier protein type 2 (SCP-2) domain of human hydroxysteroid dehydrogenase-like protein 2.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Ablating L-FABP in SCP-2/SCP-x null mice impairs bile acid metabolism and biliary HDL-cholesterol secretion.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
- Human FABP1 T94A variant impacts fatty acid metabolism and PPAR-α activation in cultured human female hepatocytes.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
- Liver fatty acid binding protein gene-ablation exacerbates weight gain in high-fat fed female mice.Lipids · 2013Article
- Inhibitors of Fatty Acid Synthesis Induce PPAR α -Regulated Fatty Acid β -Oxidative Genes: Synergistic Roles of L-FABP and Glucose.PPAR research · 2013Article
- Intracellular cholesterol-binding proteins enhance HDL-mediated cholesterol uptake in cultured primary mouse hepatocytes.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors.
Funding
Abstract
Although sterol carrier protein-2 (SCP-2) is encoded as a precursor protein (proSCP-2), little is known regarding the structure and function of the 20-amino acid N-terminal presequence. As shown herein, the presequence contains significant secondary structure and alters SCP-2: (i) secondary structure (CD), (ii) tertiary structure (aqueous exposure of Trp shown by UV absorbance, fluorescence, and fluorescence quenching), (iii) ligand binding site [Trp response to ligands, peptide cross-linked by photoactivatable free cholesterol (FCBP)], (iv) selectivity for interaction with anionic phospholipid-rich membranes, (v) interaction with a peroxisomal import protein [FRET studies of Pex5p(C) binding], the N-terminal presequence increased SCP-2's affinity for Pex5p(C) by 10-fold, and (vi) intracellular targeting in living and fixed cells (confocal microscopy). Nearly 5-fold more SCP-2 than proSCP-2 colocalized with plasma membrane lipid rafts and caveolae (AF488-CTB); 2.8-fold more SCP-2 than proSCP-2 colocalized with a mitochondrial marker (Mitotracker), but nearly 2-fold less SCP-2 than proSCP-2 colocalized with peroxisomes (AF488 antibody to PMP70). These data indicate the importance of the N-terminal presequence in regulating SCP-2 structure, cholesterol localization within the ligand binding site, membrane association, and, potentially, intracellular targeting.
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