ArticleBiological procedures online2007
Development of human gene reporter cell lines using rAAV mediated homologous recombination.
Article in Biological procedures online, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Article
- Approaches to characterize the transcriptional trajectory of human myogenesis.Cellular and molecular life sciences : CMLS · 2021Review
- Vitamin C in combination with inhibition of mutant IDH1 synergistically activates TET enzymes and epigenetically modulates gene silencing in colon cancer cells.Epigenetics · 2020Article
- Article
- IDH1-mutant cancer cells are sensitive to cisplatin and an IDH1-mutant inhibitor counteracts this sensitivity.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Clinical cancer research : an official journal of the American Association for Cancer Research · 2018Article
- Article
- Article
- Generation of double-labeled reporter cell lines for studying co-dynamics of endogenous proteins in individual human cells.PloS one · 2010Article
- Engineering of human pluripotent stem cells by AAV-mediated gene targeting.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Detection of experimentally induced pulmonary granuloma inflammation in monocyte chemoattractant protein-1 reporter mice.Molecular imaging and biology · 2010Article
- Genetic manipulation of neural progenitors derived from human embryonic stem cells.Tissue engineering. Part A · 2009Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding mechanisms of gene regulation has broad therapeutic implications for human disease. Here we describe a novel method for generating human cell lines that serve as reporters of transcriptional activity. This method exploits the ability of recombinant adeno-associated virus to mediate the insertion of exogenous DNA sequences into specific genomic loci through homologous recombination. To overcome the severe size limitation of the rAAV for carrying exogenous DNA, an enhanced green fluorescent protein (EGFP)-Luciferase fusion gene was used as both a selectable marker and gene expression reporter. EGFP was used for selection of correctly targeted alleles by taking advantage of known regulatory conditions that activate transcription of specific genes. Using this method, we describe the generation of primary human fibroblasts that express EGFP-Luciferase under the control of the c-Myc oncogene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.