ArticleThe EMBO journal2008
Ribosomal position and contacts of mRNA in eukaryotic translation initiation complexes.
Article in The EMBO journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 129 papers.
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Who cites it
129 citing papers in PubMed.
- RNAViruses · 2026Review
- Unbiased screen of human transcriptome reveals an unexpected role of 3'UTRs in translation initiation.Nature communications · 2026Article
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- Structural and molecular basis of specialized translation mediated by the ribosome mRNA-binding channel.Nature communications · 2026Article
- The intrinsic properties of mRNAs define their translation efficiency at ribosome shortage.Nucleic acids research · 2025Article
- Profiling of terminating ribosomes reveals translational control at stop codons.bioRxiv : the preprint server for biology · 2025Article
- Article
- Cellular translational enhancer elements that recruit eukaryotic initiation factor 3.RNA (New York, N.Y.) · 2025Article
- Quantitative profiling of human translation initiation reveals elements that potently regulate endogenous and therapeutically modified mRNAs.Molecular cell · 2025Article
- Functional analysis of the AUG initiator codon context reveals novel conserved sequences that disfavor mRNA translation in eukaryotes.Nucleic acids research · 2024Article
- Immunoprecipitation Methods to Isolate Messenger Ribonucleoprotein Complexes (mRNP).Advances in experimental medicine and biology · 2024Article
- Morphological and molecular phylogenetic characterization ofInternational journal for parasitology. Parasites and wildlife · 2023Article
- Depletion ofInternational journal of molecular sciences · 2023Article
- Two "Edges" in Our Knowledge on the Functions of Ribosomal Proteins: The Revealed Contributions of Their Regions to Translation Mechanisms and the Issues of Their Extracellular Transport by Exosomes.International journal of molecular sciences · 2023Review
- The Tox Gene Encodes Two Proteins with Distinct and Shared Roles in Gene Regulation.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Selective translational control of cellular and viral mRNAs by RPS3 mRNA binding.Nucleic acids research · 2023Article
- Translation regulation of specific mRNAs by RPS26 C-terminal RNA-binding tail integrates energy metabolism and AMPK-mTOR signaling.Nucleic acids research · 2023Article
- The coordinated management of ribosome and translation during injury and regeneration.Frontiers in cell and developmental biology · 2023Review
- Preinitiation Complex Loading onto mRNAs with Long versus Short 5' TLs.International journal of molecular sciences · 2022Article
69 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
The position of mRNA on 40S ribosomal subunits in eukaryotic initiation complexes was determined by UV crosslinking using mRNAs containing uniquely positioned 4-thiouridines. Crosslinking of mRNA positions (+)11 to ribosomal protein (rp) rpS2(S5p) and rpS3(S3p), and (+)9-(+)11 and (+)8-(+)9 to h18 and h34 of 18S rRNA, respectively, indicated that mRNA enters the mRNA-binding channel through the same layers of rRNA and proteins as in prokaryotes. Upstream of the P-site, the proximity of positions (-)3/(-)4 to rpS5(S7p) and h23b, (-)6/(-)7 to rpS14(S11p), and (-)8-(-)11 to the 3'-terminus of 18S rRNA (mRNA/rRNA elements forming the bacterial Shine-Dalgarno duplex) also resembles elements of the bacterial mRNA path. In addition to these striking parallels, differences between mRNA paths included the proximity in eukaryotic initiation complexes of positions (+)7/(+)8 to the central region of h28, (+)4/(+)5 to rpS15(S19p), and (-)6 and (-)7/(-)10 to eukaryote-specific rpS26 and rpS28, respectively. Moreover, we previously determined that eukaryotic initiation factor2alpha (eIF2alpha) contacts position (-)3, and now report that eIF3 interacts with positions (-)8-(-)17, forming an extension of the mRNA-binding channel that likely contributes to unique aspects of eukaryotic initiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.