ArticleProceedings of the National Academy of Sciences of the United States of America1991
Direct amplification of a single dissected chromosomal segment by polymerase chain reaction: a human brain sodium channel gene is on chromosome 2q22-q23.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 19 citations in OpenAlex.
- A second locus for familial generalized epilepsy with febrile seizures plus maps to chromosome 2q21-q33.American journal of human genetics · 1999Article
- Targeted gene walking by low stringency polymerase chain reaction: assignment of a putative human brain sodium channel gene (SCN3A) to chromosome 2q24-31.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Chromosomal assignment of seven rat homeobox genes to rat chromosomes 3, 4, 7, and 10.Mammalian genome : official journal of the International Mammalian Genome Society · 1993Article
- Primary structure, chromosomal localization, and functional expression of a voltage-gated sodium channel from human brain.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Band-specific localization of the microsatellite at D13S71 by microdissection and enzymatic amplification.American journal of human genetics · 1992Article
- The alpha-subunit of the skeletal muscle sodium channel is encoded proximal to Tk-1 on mouse chromosome 11.Mammalian genome : official journal of the International Mammalian Genome Society · 1992Article
- New nucleotide sequence data on the EMBL File Server.Nucleic acids research · 1991Article
- Structure, function and expression of voltage-dependent sodium channels.Molecular neurobiologyReview
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have devised a general strategy for gene mapping based upon the direct amplification of a target sequence within a single microdissected Giemsa-banded chromosomal segment using the polymerase chain reaction. The usefulness of this approach was demonstrated by mapping a cloned human brain sodium channel (alpha subunit) gene sequence to chromosome 2q22-q23. When DNA from single, dissected chromosome segments 2q21-qter and 2q24-pter were used as templates, a sodium channel-specific 172-base-pair polymerase chain reaction product was obtained. This product was not synthesized when segments 2q21-pter and 2q24-qter were used. Chromosome microdissection-polymerase chain reaction is not only a simple, fast, and accurate method for gene mapping but also may offer significant advantages for other applications, such as cancer cytogenetics and linkage analysis.
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