ArticleMicrocirculation (New York, N.Y. : 1994)2008
Spatial regulation of inflammation by human aortic endothelial cells in a linear gradient of shear stress.
Article in Microcirculation (New York, N.Y. : 1994), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 85 citations in OpenAlex.
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- Mechanoregulation of p38 activity enhances endoplasmic reticulum stress-mediated inflammation by arterial endothelium.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis.APL bioengineering · 2018Article
- Atherosusceptible Shear Stress Activates Endoplasmic Reticulum Stress to Promote Endothelial Inflammation.Scientific reports · 2017Article
- Targeting cell adhesion molecules with nanoparticles using in vivo and flow-based in vitro models of atherosclerosis.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- Development of a Glycosaminoglycan Derived, Selectin Targeting Anti-Adhesive Coating to Treat Endothelial Cell Dysfunction.Pharmaceuticals (Basel, Switzerland) · 2017Article
- Multiplexed Fluid Flow Device to Study Cellular Response to Tunable Shear Stress Gradients.Annals of biomedical engineering · 2016Article
- Biomimetic channel modeling local vascular dynamics of pro-inflammatory endothelial changes.Biomicrofluidics · 2016Article
- Alagebrium inhibits neointimal hyperplasia and restores distributions of wall shear stress by reducing downstream vascular resistance in obese and diabetic rats.American journal of physiology. Heart and circulatory physiology · 2015Article
- Magnetic resonance imaging-based computational modelling of blood flow and nanomedicine deposition in patients with peripheral arterial disease.Journal of the Royal Society, Interface · 2015Article
- Intima modifier locus 2 controls endothelial cell activation and vascular permeability.Physiological genomics · 2014Article
- A microfluidic shear device that accommodates parallel high and low stress zones within the same culturing chamber.Biomicrofluidics · 2014Article
- Mechanosensing at the vascular interface.Annual review of biomedical engineering · 2014Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveAtherosclerosis is a focal disease that develops at sites of low and oscillatory shear stress in arteries. This study aimed to understand how endothelial cells sense a gradient of fluid shear stress and transduce signals that regulate membrane expression of cell adhesion molecules and monocyte recruitment.
methodsHuman aortic endothelial cells were stimulated with TNF-alpha and simultaneously exposed to a linear gradient of shear stress that increased from 0 to 16 dyne/cm2. Cell adhesion molecule expression and activation of NFkappa B were quantified by immunofluorescence microscopy with resolution at the level of a single endothelial cell. Monocyte recruitment was imaged using custom microfluidic flow chambers.
resultsVCAM-1 and E-selectin upregulation was greatest between 2-4 dyne/cm2 (6 and 4-fold, respectively) and above 8 dyne/cm2 expression was suppressed below that of untreated endothelial cells. In contrast, ICAM-1 expression and NFkappa B nuclear translocation increased with shear stress up to a maximum at 9 dyne/cm2. Monocyte recruitment was most efficient in regions where E-selectin and VCAM-1 expression was greatest.
conclusionsWe found that the endothelium can sense a change in shear stress on the order of 0.25 dyne/cm2 over a length of approximately 10 cells, regulating the level of protein transcription, cellular adhesion molecule expression, and leukocyte recruitment during inflammation.
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