ArticleHistochemistry and cell biology2008
Electron microscopic visualization of fluorescent signals in cellular compartments and organelles by means of DAB-photoconversion.
Article in Histochemistry and cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- Transmission Electron Microscopy as a Powerful Tool to Investigate the Interaction of Nanoparticles with Subcellular Structures.International journal of molecular sciences · 2021Review
- Electron tomography-a tool for ultrastructural 3D visualization in cell biology and histology.Wiener medizinische Wochenschrift (1946) · 2018Review
- A fluoro-Nissl dye identifies pericytes as distinct vascular mural cells during in vivo brain imaging.Nature neuroscience · 2017Article
- Determining the target protein localization in 3D using the combination of FIB-SEM and APEX2.Biophysics reports · 2017Article
- Metallothionein as a clonable tag for protein localization by electron microscopy of cells.Journal of microscopy · 2015Article
- FluoroNanogold: an important probe for correlative microscopy.Journal of chemical biology · 2015Review
- 2-Deoxy-D-glucose treatment changes the Golgi apparatus architecture without blocking synthesis of complex lipids.Histochemistry and cell biology · 2015Article
- Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae.eLife · 2014Article
- Simultaneous ultrastructural analysis of fluorochrome-photoconverted diaminobenzidine and gold immunolabelling in cultured cells.European journal of histochemistry : EJH · 2013Article
- Photochemically Active Fluorophore-DNA/RNA Conjugates for Cellular Imaging of Nucleic Acids by Readout in Electron Microscopy.ChemistryOpen · 2013Article
- Ultrastructural detection of photosensitizing molecules by fluorescence photoconversion of diaminobenzidine.Histochemistry and cell biology · 2013Article
- Photooxidation-guided ultrastructural identification and analysis of cells in neuronal tissue labeled with green fluorescent protein.PloS one · 2013Article
- Article
- Combined light and electron microscopy using diaminobenzidine photooxidation to monitor trafficking of lipids derived from lipoprotein particles.Current pharmaceutical biotechnology · 2012Article
- Architecture of the mammalian Golgi.Cold Spring Harbor perspectives in biology · 2011Review
- Alzheimer's disease-associated ubiquilin-1 regulates presenilin-1 accumulation and aggresome formation.Traffic (Copenhagen, Denmark) · 2011Article
- The ceramide-enriched trans-Golgi compartments reorganize together with other parts of the Golgi apparatus in response to ATP-depletion.Histochemistry and cell biology · 2011Article
- Direct visualization of protein association in living cells with complex-edited electron microscopy.Angewandte Chemie (International ed. in English) · 2010Article
- Formation and maintenance of blood-urine barrier in urothelium.Protoplasma · 2010Review
- Characterization of endocytic compartments after holo-high density lipoprotein particle uptake in HepG2 cells.Histochemistry and cell biology · 2010Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
In this work, we show the photoconversion of the fluorochromes enhanced green fluorescent protein (EGFP), yellow fluorescent protein (YFP), and BODIPY into electron dense diaminobenzidine (DAB)-deposits using the examples of five different target proteins, and the lipid ceramide. High spatial resolution and specificity in the localization of the converted protein-fluorochrome complexes and the fluorochrome-labelled lipid were achieved by methodical adaptations around the DAB-photooxidation step, such as fixation, illumination, controlled DAB-precipitation, and osmium postfixation. The DAB-deposits at the plasma membrane and membranous compartments, such as endoplasmic reticulum and Golgi apparatus in combination with the fine structural preservation and high membrane contrast enabled differential topographical analyses, and allowed three-dimensional reconstructions of complex cellular architectures, such as trans-Golgi-ER junctions. On semithin sections the quality, distribution and patterns of the signals were evaluated; defined areas of interest were used for electron microscopic analyses and correlative microscopy of consecutive ultrathin sections. The results obtained with the proteins golgin 84 (G-84), protein disulfide isomerase (PDI), scavenger receptor classB type1 (SR-BI), and gamma-aminobutyric acid (GABA) transporter 1 (GAT1), on one hand closely matched with earlier immunocytochemical data and, on the other hand, led to new information about their subcellular localizations as exemplified by a completely novel sight on the subcellular distribution and kinetics of the SR-BI, and provided a major base for the forthcoming research.
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