ArticleJournal of clinical microbiology2008
Quadruplex real-time PCR assay using allele-specific scorpion primers for detection of mutations conferring clarithromycin resistance to Helicobacter pylori.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 69 citations in OpenAlex.
- Characterization using ultra-deep sequencing of the intra-host distribution of the mutations associated with H. pylori antibiotic resistance.Annals of clinical microbiology and antimicrobials · 2026Article
- Comparative analysis of the detection of antibiotic genotypic resistance with gastric mucosa, gastric fluid, and fecal samples in patients withJournal of clinical microbiology · 2025Article
- Development and Validation of Multiplex Quantitative PCR Assay for Detection ofInfection and drug resistance · 2021Article
- Validation of loop-mediated isothermal amplification to detect Helicobacter pylori and 23S rRNA mutations: A prospective, observational clinical cohort study.Journal of clinical laboratory analysis · 2021Article
- Where to Biopsy to DetectJournal of clinical medicine · 2020Article
- Diagnostic Accuracy of a Noninvasive Test for Detection of Helicobacter pylori and Resistance to Clarithromycin in Stool by the Amplidiag H. pylori+ClariR Real-Time PCR Assay.Journal of clinical microbiology · 2020Article
- Trends inMicrobial genomics · 2020Review
- The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori.Revista do Instituto de Medicina Tropical de Sao Paulo · 2018Article
- Review article: the global emergence of Helicobacter pylori antibiotic resistance.Alimentary pharmacology & therapeutics · 2016Review
- Helicobacter pylori infection - recent developments in diagnosis.World journal of gastroenterology · 2014Review
- Chemokines and antimicrobial peptides have a cag-dependent early response to Helicobacter pylori infection in primary human gastric epithelial cells.Infection and immunity · 2014Article
- The relationship between gastric cancer and Helicobacter pylori in formaldehyde fixed paraffin embedded gastric tissues of gastric cancer patients-scorpion real-time PCR assay findings.Pathology oncology research : POR · 2014Article
- Low Helicobacter pylori primary resistance to clarithromycin in gastric biopsy specimens from dyspeptic patients of a city in the interior of São Paulo, Brazil.BMC gastroenterology · 2013Article
- Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of Helicobacter pylori clarithromycin resistance in gastric biopsy specimens.Journal of clinical microbiology · 2013Article
- Absence of Helicobacter pylori high tetracycline resistant 16S rDNA AGA926-928TTC genotype in gastric biopsy specimens from dyspeptic patients of a city in the interior of São Paulo, Brazil.BMC gastroenterology · 2012Article
- Agreement rate of rapid urease test, conventional PCR, and scorpion real-time PCR in detecting helicobacter pylori from tonsillar samples of patients with chronic tonsillitis.Journal of global infectious diseases · 2012Article
- State of the globe: Diagnostic tests to detect Helicobacter pylori tonsillitis.Journal of global infectious diseases · 2012Article
- Assessment of helicobacter pylori prevalence by scorpion real-time PCR in chronic tonsillitis patients.Journal of global infectious diseases · 2012Article
- Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates.Journal of clinical microbiology · 2011Article
- DPO multiplex PCR as an alternative to culture and susceptibility testing to detect Helicobacter pylori and its resistance to clarithromycin.BMC gastroenterology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a single-vessel multiplex real-time PCR assay that detects Helicobacter pylori infection and identified the four existing alleles of the 23S rRNA genes of H. pylori--the wild-type sequence and the three mutations conferring clarithromycin resistance--using allele-specific Scorpion primers directly on biopsy specimens. The Scorpion primers combine a primer and a probe in a single molecule and are able to distinguish single-nucleotide polymorphism. Fluorescent signals, produced when the probes are annealed, are read in four channels by a SmartCycler thermocycler. The assay was first applied successfully on 4 reference and 61 clinical strains. MICs of clarithromycin were determined by the Etest method. A perfect concordance was obtained between Etest and Scorpion PCR. Mixed populations were better detected by Scorpion PCR. We examined 259 biopsies from 229 patients by culture, PCR-restriction fragment length polymorphism (RFLP), and Scorpion PCR. One biopsy, positive for culture, exhibited inhibitors for both PCR-RFLP and Scorpion PCR. Twelve biopsies were positive for PCR-RFLP and Scorpion PCR but negative for culture with concordant determination of mutations in the 23S rRNA genes by the two PCR assays. Three biopsies were positive for Scorpion PCR only. Compared to culture, the sensitivity of Scorpion PCR was 98.3% and the specificity was 92.5%. The Scorpion PCR assay provides a highly accurate, rapid, and precise method for the detection and determination of mutations conferring clarithromycin resistance to H. pylori.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.