Evidence map›Paper›PMID 18463206›Full record

ArticleJournal of clinical microbiology2008

Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samples.

Kwok Hung Chan, Wing Cheong Yam, Chiu Mei Pang, Kit Man Chan, Siu Yan Lam, Kam Fai Lo, Leo L M Poon, J S Malik Peiris

Open access · greenAbstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
4.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 49 citations in OpenAlex.

  1. Review
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  5. Comparison of eMAG™ versus NucliSENSJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2017
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  12. Automated, simple, and efficient influenza RNA extraction from clinical respiratory swabs using TruTip and epMotion.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2013
    Article
  13. Article
  14. Article
  15. Optimization of Toxoplasma gondii DNA extraction from amniotic fluid using NucliSENS easyMAG and comparison with QIAamp DNA minikit.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Kwok Hung ChanDepartment of Microbiology, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong, SAR, People's Republic of China.
Wing Cheong Yam
Chiu Mei Pang
Kit Man Chan
Siu Yan Lam
Kam Fai Lo
Leo L M Poon
J S Malik Peiris
Queen Mary Hospital · CNUniversity of Hong Kong · HK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The NucliSens easyMAG and BioRot 9604 automated nucleic acid extraction systems were evaluated and compared with the manual QIAamp (Qiagen) extraction method for their abilities to extract nucleic acid from nasopharyngeal aspirate samples for the detection of RNA and DNA respiratory viruses. The nucleic acids recovered by all three methods gave comparable sensitivities in PCR tests, and the three methods gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination.

Indexed as

AutomationDNA, ViralDNA VirusesHong KongHospitalsHumansNasopharynxPolymerase Chain ReactionRNA, ViralRNA VirusesSensitivity and SpecificityVirologyVirus DiseasesDNA, ViralRNA, Viral

Identifiers

PMID18463206
PMCPMC2446927
OpenAlexW1987693053

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.