ArticleMolecular biology of the cell2008
High-resolution crystal structure and in vivo function of a kinesin-2 homologue in Giardia intestinalis.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 56 citations in OpenAlex.
- The cytoskeleton of pathogenic protists.Bioscience reports · 2026Review
- Review
- Regulation of kinesin-2 motility by its β-hairpin motif.Nature structural & molecular biology · 2025Article
- Article
- Giardia intraflagellar transport protein 88 is involved in flagella formation.Parasites, hosts and diseases · 2025Article
- A polo-like kinase modulates cytokinesis and flagella biogenesis in Giardia lamblia.Parasites & vectors · 2021Article
- Structural snapshots of the kinesin-2 OSM-3 along its nucleotide cycle: implications for the ATP hydrolysis mechanism.FEBS open bio · 2021Article
- How Kinesin-1 Utilize the Energy of Nucleotide: The Conformational Changes and Mechanochemical Coupling in the Unidirectional Motion of Kinesin-1.International journal of molecular sciences · 2020Review
- mRNA localization mediates maturation of cytoplasmic cilia in Drosophila spermatogenesis.The Journal of cell biology · 2020Article
- Nek8445, a protein kinase required for microtubule regulation and cytokinesis inMolecular biology of the cell · 2020Article
- Recent advances in functional research in Giardia intestinalis.Advances in parasitology · 2020Review
- Microtubule organelles in Giardia.Advances in parasitology · 2020Review
- Article
- A Maternal Transcription Factor, Junction Mediating and Regulatory Protein is Required for Preimplantation Development in the Mouse.Development & reproduction · 2019Article
- The role of satellite and other functional cell types in muscle repair and regeneration.Journal of muscle research and cell motility · 2019Review
- Robust and stable transcriptional repression in Giardia using CRISPRi.Molecular biology of the cell · 2019Article
- Article
- Review
- Shared and Distinct Mechanisms of Compartmentalized and Cytosolic Ciliogenesis.Current biology : CB · 2015Review
- The Critical Role of the Cytoskeleton in the Pathogenesis ofCurrent clinical microbiology reports · 2015Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
A critical component of flagellar assembly, the kinesin-2 heterotrimeric complex powers the anterograde movement of proteinaceous rafts along the outer doublet of axonemes in intraflagellar transport (IFT). We present the first high-resolution structures of a kinesin-2 motor domain and an ATP hydrolysis-deficient motor domain mutant from the parasitic protist Giardia intestinalis. The high-resolution crystal structures of G. intestinalis wild-type kinesin-2 (GiKIN2a) motor domain, with its docked neck linker and the hydrolysis-deficient mutant GiKIN2aT104N were solved in a complex with ADP and Mg(2+) at 1.6 and 1.8 A resolutions, respectively. These high-resolution structures provide unique insight into the nucleotide coordination within the active site. G. intestinalis has eight flagella, and we demonstrate that both kinesin-2 homologues and IFT proteins localize to both cytoplasmic and membrane-bound regions of axonemes, with foci at cell body exit points and the distal flagellar tips. We demonstrate that the T104N mutation causes GiKIN2a to act as a rigor mutant in vitro. Overexpression of GiKIN2aT104N results in significant inhibition of flagellar assembly in the caudal, ventral, and posterolateral flagellar pairs. Thus we confirm the conserved evolutionary structure and functional role of kinesin-2 as the anterograde IFT motor in G. intestinalis.
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