ArticleMolecular biology of the cell2008
Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 66 citations in OpenAlex.
- The multitalented TIP60 chromatin remodeling complex: wearing many hats in epigenetic regulation, cell division and diseases.Epigenetics & chromatin · 2025Review
- The multi-faceted roles of R2TP complex span across regulation of gene expression, translation, and protein functional assembly.Biophysical reviews · 2023Review
- Unconventional roles of chromatin remodelers and long non-coding RNAs in cell division.Cellular and molecular life sciences : CMLS · 2023Review
- Downregulation of AHNAK2 inhibits cell cycle of lung adenocarcinoma cells by interacting with RUVBL1.Thoracic cancer · 2023Article
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- The Green Valley ofCells · 2022Review
- Evolutionary conserved relocation of chromatin remodeling complexes to the mitotic apparatus.BMC biology · 2022Article
- Review
- The ATPase SRCAP is associated with the mitotic apparatus, uncovering novel molecular aspects of Floating-Harbor syndrome.BMC biology · 2021Article
- Assembly of the asymmetric human γ-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase.Science advances · 2020Article
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- Article
- Quantifying post-transcriptional regulation in the development of Drosophila melanogaster.Nature communications · 2018Article
- Targeted deletion of the AAA-ATPase Ruvbl1 in mice disrupts ciliary integrity and causes renal disease and hydrocephalus.Experimental & molecular medicine · 2018Article
- Potential mechanism and drug candidates for sepsis-induced acute lung injury.Experimental and therapeutic medicine · 2018Article
- Genome maintenance functions of the INO80 chromatin remodeller.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- The Role of Pontin and Reptin in Cellular Physiology and Cancer Etiology.Frontiers in molecular biosciences · 2017Review
- Specific threonine-4 phosphorylation and function of RNA polymerase II CTD during M phase progression.Scientific reports · 2016Article
- A genetic interaction map of cell cycle regulators.Molecular biology of the cell · 2016Article
- Chromosome Missegregation Associated with RUVBL1 Deficiency.PloS one · 2015Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
To identify novel proteins important for microtubule assembly in mitosis, we have used a centrosome-based complementation assay to enrich for proteins with mitotic functions. An RNA interference (RNAi)-based screen of these proteins allowed us to uncover 13 novel mitotic regulators. We carried out in-depth analyses of one of these proteins, Pontin, which is known to have several functions in interphase, including chromatin remodeling, DNA repair, and transcription. We show that reduction of Pontin by RNAi resulted in defects in spindle assembly in Drosophila S2 cells and in several mammalian tissue culture cell lines. Further characterization of Pontin in Xenopus egg extracts demonstrates that Pontin interacts with the gamma tubulin ring complex (gamma-TuRC). Because depletion of Pontin leads to defects in the assembly and organization of microtubule arrays in egg extracts, our studies suggest that Pontin has a mitosis-specific function in regulating microtubule assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.