ArticleJournal of virology2008
Role of initiating nucleoside triphosphate concentrations in the regulation of influenza virus replication and transcription.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
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- Deformed wing virus coopts the host arginine kinase to enhance its fitness in honey bees (Apis mellifera).BMC biology · 2025Article
- Mathematical model calibrated toiScience · 2024Article
- Review
- Rhinovirus C causes heterogeneous infection and gene expression in airway epithelial cell subsets.Mucosal immunology · 2023Article
- RNA Overwriting of Cellular mRNA by Cas13b-Directed RNA-Dependent RNA Polymerase of Influenza A Virus.International journal of molecular sciences · 2023Article
- The Host Factor ANP32A Is Required for Influenza A Virus vRNA and cRNA Synthesis.Journal of virology · 2022Article
- Mechanistic cross-talk between DNA/RNA polymerase enzyme kinetics and nucleotide substrate availability in cells: Implications for polymerase inhibitor discovery.The Journal of biological chemistry · 2020Review
- ATeam technology for detecting early signs of viral cytopathic effect.The Journal of veterinary medical science · 2020Article
- A Novel Type of Influenza A Virus-Derived Defective Interfering Particle with Nucleotide Substitutions in Its Genome.Journal of virology · 2019Article
- Influenza A virus inhibits influenza virus replication by inducing IL-37.Journal of clinical laboratory analysis · 2019Article
- Role of the PB2 627 Domain in Influenza A Virus Polymerase Function.Journal of virology · 2017Article
- A Nucleolar Protein, Ribosomal RNA Processing 1 Homolog B (RRP1B), Enhances the Recruitment of Cellular mRNA in Influenza Virus Transcription.Journal of virology · 2015Article
- Crucial role of PA in virus life cycle and host adaptation of influenza A virus.Medical microbiology and immunology · 2015Review
- The RNA-dependent RNA polymerase of the influenza A virus.Future virology · 2014Article
- Deep sequencing reveals the eight facets of the influenza A/HongKong/1/1968 (H3N2) virus cap-snatching process.Scientific reports · 2014Article
- Adaptation of avian influenza A virus polymerase in mammals to overcome the host species barrier.Journal of virology · 2013Review
- A small-RNA enhancer of viral polymerase activity.Journal of virology · 2012Article
- Recombinant influenza A viruses with enhanced levels of PB1 and PA viral protein expression.Journal of virology · 2012Article
- Visualization and measurement of ATP levels in living cells replicating hepatitis C virus genome RNA.PLoS pathogens · 2012Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The mechanisms regulating the synthesis of mRNA, cRNA, and viral genomic RNA (vRNA) by the influenza A virus RNA-dependent RNA polymerase are not fully understood. Previous studies in our laboratory have shown that virion-derived viral ribonucleoprotein complexes synthesize both mRNA and cRNA in vitro and early in the infection cycle in vivo. Our continued studies showed that de novo synthesis of cRNA in vitro is more sensitive to the concentrations of ATP, CTP, and GTP than capped-primer-dependent synthesis of mRNA. Using rescued recombinant influenza A/WSN/33 viruses, we now demonstrate that the 3'-terminal sequence of the vRNA promoter dictates the requirement for a high nucleoside triphosphate (NTP) concentration during de novo-initiated replication to cRNA, whereas this is not the case for the extension of capped primers during transcription to mRNA. In contrast to some other viral polymerases, for which only the initiating NTP is required at high concentrations, influenza virus polymerase requires high concentrations of the first three NTPs. In addition, we show that base pair mutations in the vRNA promoter can lead to nontemplated dead-end mutations during replication to cRNA in vivo. Based on our observations, we propose a new model for the de novo initiation of influenza virus replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.