Evidence map›Paper›PMID 18462507›Full record

ArticleBMC microbiology2008

Performance of a 70-mer oligonucleotide microarray for genotyping of Campylobacter jejuni.

Sandra Rodin, Anders F Andersson, Valtteri Wirta, Lena Eriksson, Marianne Ljungström, Britta Björkholm, Hans Lindmark, Lars Engstrand

Open access · goldAbstract readEvaluation Study
In one paragraph

Article in BMC microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
6.1field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Sandra RodinSwedish Institute for Infectious Disease Control, SE-17182 Solna, Sweden. sandra.rodin@smi.ki.se
Anders F Andersson
Valtteri Wirta
Lena Eriksson
Marianne Ljungström
Britta Björkholm
Hans Lindmark
Lars Engstrand
Swedish Institute · SEKarolinska Institutet · SENational Food Administration · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCampylobacter jejuni is widespread in the environment and is the major cause of bacterial gastroenteritis in humans. In the present study we use microarray-based comparative genomic hybridizations (CGH), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) to analyze closely related C. jejuni isolates from chicken and human infection.

resultsWith the exception of one isolate, the microarray data clusters the isolates according to the five groups determined by PFGE. In contrast, MLST defines only three genotypes among the isolates, indicating a lower resolution. All methods show that there is no inherit difference between isolates infecting humans and chicken, suggesting a common underlying population of C. jejuni. We further identify regions that frequently differ between isolates, including both previously described and novel regions. Finally, we show that genes that belong to certain functional groups differ between isolates more often than expected by chance.

conclusionIn this study we demonstrated the utility of 70-mer oligonucleotide microarrays for genotyping of Campylobacter jejuni isolates, with resolution outperforming MLST.

Indexed as

AnimalsBacterial Typing TechniquesCampylobacter InfectionsCampylobacter jejuniChickensDNA, BacterialElectrophoresis, Gel, Pulsed-FieldGenotypeHumansNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisPoultry DiseasesSequence Analysis, DNADNA, Bacterial

Identifiers

PMID18462507
PMCPMC2396164
OpenAlexW2071673014

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.