ArticlePLoS biology2008
Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated.
Article in PLoS biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 132 citations in OpenAlex.
- Identification of feeding apparatus components in a heterotrophic marine flagellate.Journal of cell science · 2026Article
- Polo-like kinase phosphorylation of the orphan kinesin KIN-G negatively regulates centrin arm biogenesis ineLife · 2026Article
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- The subcellular architecture ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Visualization of Trypanosoma brucei flagellar pocket collar biogenesis identifies two new cytoskeletal structures.PLoS biology · 2025Article
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- An orphan kinesin inmBio · 2024Article
- LRRC56 is an IFT cargo required for assembly of the distal dynein docking complex inMolecular biology of the cell · 2024Article
- Characterisation of TbSmee1 suggests endocytosis allows surface-bound cargo to enter the trypanosome flagellar pocket.Journal of cell science · 2023Article
- FAZ assembly in bloodstream formMolecular biology of the cell · 2023Article
- TbKINX1B: a novel BILBO1 partner and an essential protein in bloodstream form Trypanosoma brucei.Parasite (Paris, France) · 2022Article
- Bhalin, an Essential Cytoskeleton-Associated Protein ofMicroorganisms · 2021Article
- Intrabody-Induced Cell Death by Targeting the T. brucei Cytoskeletal ProteinMicrobiology spectrum · 2021Article
- Structural and functional studies of the first tripartite protein complex at the Trypanosoma brucei flagellar pocket collar.PLoS pathogens · 2021Article
- The kinesin of the flagellum attachment zone in Leishmania is required for cell morphogenesis, cell division and virulence in the mammalian host.PLoS pathogens · 2021Article
- A specific basal body linker protein provides the connection function for basal body inheritance in trypanosomes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- To the Surface and Back: Exo- and Endocytic Pathways inFrontiers in cell and developmental biology · 2021Review
- Polo-like kinase in trypanosomes: an odd member out of the Polo family.Open biology · 2020Article
- Maintenance of hook complex integrity and centrin arm assembly facilitates flagellum inheritance inThe Journal of biological chemistry · 2020Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trypanosoma brucei is a protozoan parasite that is used as a model organism to study such biological phenomena as gene expression, protein trafficking, and cytoskeletal biogenesis. In T. brucei, endocytosis and exocytosis occur exclusively through a sequestered organelle called the flagellar pocket (FP), an invagination of the pellicular membrane. The pocket is the sole site for specific receptors thus maintaining them inaccessible to components of the innate immune system of the mammalian host. The FP is also responsible for the sorting of protective parasite glycoproteins targeted to, or recycling from, the pellicular membrane, and for the removal of host antibodies from the cell surface. Here, we describe the first characterisation of a flagellar pocket cytoskeletal protein, BILBO1. BILBO1 functions to form a cytoskeleton framework upon which the FP is made and which is also required and essential for FP biogenesis and cell survival. Remarkably, RNA interference (RNAi)-mediated ablation of BILBO1 in insect procyclic-form parasites prevents FP biogenesis and induces vesicle accumulation, Golgi swelling, the aberrant repositioning of the new flagellum, and cell death. Cultured bloodstream-form parasites are also nonviable when subjected to BILBO1 RNAi. These results provide the first molecular evidence for cytoskeletally mediated FP biogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.