ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2008
Dynamics of the CapG actin-binding protein in the cell nucleus studied by FRAP and FCS.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Macrophage capping protein CapG is a putative oncogene involved in migration and invasiveness in ovarian carcinoma.BioMed research international · 2014Trial
- Repeated FRAP of the actin-binding protein CapG in the cell nucleus-a functional assay for EGF signaling in the single live breast cancer cell.Scientific reports · 2024Article
- Experimentally Probing the Effect of Confinement Geometry on Lipid Diffusion.The journal of physical chemistry. B · 2024Article
- Capping Actin Protein Overexpression in Human Colorectal Carcinoma and Its Contributed Tumor Migration.Analytical cellular pathology (Amsterdam) · 2018Article
- Function of the macrophage-capping protein in colorectal carcinoma.Oncology letters · 2017Article
- By moonlighting in the nucleus, villin regulates epithelial plasticity.Molecular biology of the cell · 2016Article
- Viscosity-dependent diffusion of fluorescent particles using fluorescence correlation spectroscopy.Journal of fluorescence · 2014Article
- Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy.PloS one · 2012Article
- 2D difference gel electrophoresis analysis of different time points during the course of neoplastic transformation of human mammary epithelial cells.Journal of proteome research · 2011Article
- Cross-validating FRAP and FCS to quantify the impact of photobleaching on in vivo binding estimates.Biophysical journal · 2010Article
- FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know?Current opinion in cell biology · 2010Review
- Direct measurement of association and dissociation rates of DNA binding in live cells by fluorescence correlation spectroscopy.Biophysical journal · 2009Article
- The long journey of actin and actin-associated proteins from genes to polysomes.Cellular and molecular life sciences : CMLS · 2009Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
FRAP (fluorescence recovery after photobleaching) and FCS (fluorescence correlation spectroscopy) are spectroscopic methods for monitoring the dynamic distribution of proteins inside the nucleus of living cells. As an example we report our studies on the intracellular mobility of the actin-binding protein CapG in live breast cancer cells. This Gelsolin-related protein is a putative oncogene. It appears to be overexpressed especially in metastasizing breast cancer. Furthermore, the CapG protein is known to be involved in the motility control of non-muscle benign cells. Its increased expression triggers an increase in cell motility of benign cells. Thus it can be expected that in cancer cells overexpressing the CapG protein, motility, invasiveness and metastasis might be particularly promoted. Since the nuclear CapG fraction seems to be pivotal to the increase in cell motility, we focused our studies on the CapG mobility in cell nuclei of live breast cancer cells. Using FCS and FRAP we showed that the eGFP-tagged CapG is monomeric and characterized its diffusional properties on the microsecond to minute timescale. This information about the mobility and compartmentalization of CapG might help to provide insight into its function within the cell nucleus and give clues about its altered cellular function in malignant dedifferentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.