ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2008
Changes in chromosome organization during PHA-activation of resting human lymphocytes measured by cryo-FISH.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Epigenetic regulatory layers in the 3D nucleus.Molecular cell · 2024Review
- Elucidating the structure and function of the nucleus-The NIH Common Fund 4D Nucleome program.Molecular cell · 2023Review
- 3D chromatin architecture and transcription regulation in cancer.Journal of hematology & oncology · 2022Review
- Altered nuclear architecture in blood cells from Huntington's disease patients.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2022Article
- Review
- Multi-contact 3C reveals that the human genome during interphase is largely not entangled.Nature structural & molecular biology · 2020Article
- Methods for mapping 3D chromosome architecture.Nature reviews. Genetics · 2020Review
- Radial Organization in the Mammalian Nucleus.Frontiers in genetics · 2020Review
- Polymer Modeling Predicts Chromosome Reorganization in Senescence.Cell reports · 2019Article
- Three-dimensional genome structures of single diploid human cells.Science (New York, N.Y.) · 2018Article
- Article
- Article
- Understanding Spatial Genome Organization: Methods and Insights.Genomics, proteomics & bioinformatics · 2016Review
- Three-dimensional genome architecture: players and mechanisms.Nature reviews. Molecular cell biology · 2015Review
- Myosin VI regulates gene pairing and transcriptional pause release in T cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Chromosome territory repositioning induced by PHA-activation of lymphocytes: A 2D and 3D appraisal.Molecular cytogenetics · 2015Article
- A Crowdsourced nucleus: understanding nuclear organization in terms of dynamically networked protein function.Biochimica et biophysica acta · 2014Review
- 3D chromatin conformation correlates with replication timing and is conserved in resting cells.Nucleic acids research · 2012Article
- Non-random organization of the Biomphalaria glabrata genome in interphase Bge cells and the spatial repositioning of activated genes in cells co-cultured with Schistosoma mansoni.International journal for parasitology · 2011Article
- Innate structure of DNA foci restricts the mixing of DNA from different chromosome territories.PloS one · 2011Article
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4 authors.
Funding
Abstract
During interphase, chromosomes are arranged into territories within a highly organized nuclear space containing several compartments. It is becoming clear that this complex nuclear arrangement is important for gene regulation and therefore expression. The study of chromosome organization in interphase requires high-resolution imaging methods that at the same time allow for flexible labelling strategies and preserve nuclear structure. Tokuyasu cryosections of cells or tissues provide a simple, high-resolution platform for performing immunolabelling and fluorescence in situ hybridization (FISH) on well-preserved samples. Here we show how FISH performed on thin cryosections (cryo-FISH) can be used for the study of chromosome organization at high resolution and in a quantitative manner. We have measured chromosome intermingling, volume and radial position, in resting and activated human lymphocytes, and observed chromosome-specific differences between the two cellular states. These differences are in part related to the nuclear expansion that occurs during activation, but are also likely to be tied to their different transcriptional profiles. Extrapolation of our dataset to the whole genome suggests that activated cells contain a lower amount of chromatin involved in intermingling than resting cells.
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