ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2008
High-precision structural analysis of subnuclear complexes in fixed and live cells via spatially modulated illumination (SMI) microscopy.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Biophysics is reshaping our perception of the epigenome: from DNA-level to high-throughput studies.Biophysical reports · 2021Review
- Super-Resolution Microscopy of Chromatin.Genes · 2019Review
- Emerging views of the nucleus as a cellular mechanosensor.Nature cell biology · 2018Review
- Super-resolution binding activated localization microscopy through reversible change of DNA conformation.Nucleus (Austin, Tex.) · 2018Review
- Quantitative super-resolution localization microscopy of DNA in situ using Vybrant® DyeCycle™ Violet fluorescent probe.Data in brief · 2016Article
- Subnuclear localization, rates and effectiveness of UVC-induced unscheduled DNA synthesis visualized by fluorescence widefield, confocal and super-resolution microscopy.Cell cycle (Georgetown, Tex.) · 2016Article
- Superresolution imaging reveals structurally distinct periodic patterns of chromatin along pachytene chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Quantification of Internalized Silica Nanoparticles via STED Microscopy.BioMed research international · 2015Article
- Application perspectives of localization microscopy in virology.Histochemistry and cell biology · 2014Article
- Advanced fluorescence microscopy techniques--FRAP, FLIP, FLAP, FRET and FLIM.Molecules (Basel, Switzerland) · 2012Review
- Localization microscopy (SPDM) reveals clustered formations of P-glycoprotein in a human blood-brain barrier model.PloS one · 2012Article
- Combining FISH with localisation microscopy: Super-resolution imaging of nuclear genome nanostructures.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Monte Carlo Simulations indicate that Chromati: Nanostructure is accessible by Light Microscopy.PMC biophysics · 2010Article
- Measurement of replication structures at the nanometer scale using super-resolution light microscopy.Nucleic acids research · 2010Article
- Dynamics of telomeres and promyelocytic leukemia nuclear bodies in a telomerase-negative human cell line.Molecular biology of the cell · 2009Article
- Single molecule localization microscopy of the distribution of chromatin using Hoechst and DAPI fluorescent probes.Nucleus (Austin, Tex.)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spatially modulated illumination (SMI) microscopy is a method of wide field fluorescence microscopy featuring interferometric illumination, which delivers structural information about nanoscale architecture in fluorescently labelled cells. The first prototype of the SMI microscope proved its applicability to a wide range of biological questions. For the SMI live cell imaging this system was enhanced in terms of the development of a completely new upright configuration. This so called Vertico-SMI transfers the advantages of SMI nanoscaling to vital biological systems, and is shown to work consistently at different temperatures using both oil- and water-immersion objective lenses. Furthermore, we increased the speed of data acquisition to minimize errors in the detection signal resulting from cellular or object movement. By performing accurate characterization, the present Vertico-SMI now offers a fully-fledged microscope enabling a complete three-dimensional (3D) SMI data stack to be acquired in less than 2 seconds. We have performed live cell measurements of a tet-operator repeat insert in U2OS cells, which provided the first in vivo signatures of subnuclear complexes. Furthermore, we have successfully implemented an optional optical configuration allowing the generation of high-resolution localization microscopy images of a nuclear pore complex distribution.
Indexed as
Identifiers
18461478What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.