ArticleClinical & experimental metastasis2008
Rho GTPases in PC-3 prostate cancer cell morphology, invasion and tumor cell diapedesis.
Article in Clinical & experimental metastasis, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 69 citations in OpenAlex.
- Proteomic Analysis of Prostate Cancer FFPE Samples Reveals Markers of Disease Progression and Aggressiveness.Cancers · 2022Article
- Role of RhoC in cancer cell migration.Cancer cell international · 2021Review
- The Effect of Ras Homolog C/Rho-Associated Coiled-Protein Kinase (Rho/ROCK) Signaling Pathways on Proliferation and Apoptosis of Human Myeloma Cells.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- Article
- Role of the lncRNA ABHD11-ASMolecular cancer · 2017Article
- Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq.Science (New York, N.Y.) · 2017Article
- Genetic variants in RhoA and ROCK1 genes are associated with the development, progression and prognosis of prostate cancer.Oncotarget · 2017Article
- Small-Molecule Inhibition of Rho/MKL/SRF Transcription in Prostate Cancer Cells: Modulation of Cell Cycle, ER Stress, and Metastasis Gene Networks.Microarrays (Basel, Switzerland) · 2016Article
- Quantification of cancer cell extravasation in vivo.Nature protocols · 2016Article
- MicroRNA-372 inhibits endometrial carcinoma development by targeting the expression of the Ras homolog gene family member C (RhoC).Oncotarget · 2016Article
- Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network.Oncotarget · 2015Article
- RhoC maintains vascular homeostasis by regulating VEGF-induced signaling in endothelial cells.Journal of cell science · 2015Article
- Approaches of targeting Rho GTPases in cancer drug discovery.Expert opinion on drug discovery · 2015Review
- ROBO1, a tumor suppressor and critical molecular barrier for localized tumor cells to acquire invasive phenotype: study in African-American and Caucasian prostate cancer models.International journal of cancer · 2014Article
- The role of RhoC in epithelial-to-mesenchymal transition of ovarian carcinoma cells.BMC cancer · 2014Article
- DLC1 induces expression of E-cadherin in prostate cancer cells through Rho pathway and suppresses invasion.Oncogene · 2014Article
- Identification of beta-2 as a key cell adhesion molecule in PCa cell neurotropic behavior: a novel ex vivo and biophysical approach.PloS one · 2014Article
- Crossing the endothelial barrier during metastasis.Nature reviews. Cancer · 2013Review
- Article
- Geranylgeranyltransferase I promotes human glioma cell growth through Rac1 membrane association and activation.Journal of molecular neuroscience : MN · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Rho GTPases comprise one of the eight subfamilies of the Ras superfamily of monomeric GTP-binding proteins and are involved in cytoskeletal organization. Previously, using a dominant negative construct, we demonstrated a role for RhoC GTPase in conferring invasive capabilities to PC-3 human prostate cancer cells. Further, we demonstrated that inactivation of RhoC led to morphological changes commensurate with epithelial to mesenchymal transition (EMT) and was accompanied by increased random, linear motility and decreased directed migration and invasion. EMT was related positively to sustained expression and activity of Rac GTPase. In the current study we analyze the individual roles of RhoA, RhoC and Rac1 GTPases in PC-3 cell directed migration, invasion and tumor cell diapedesis across a human bone marrow endothelial cell layer in vitro.
resultsUse of specific shRNA directed against RhoA, RhoC or Rac1 GTPases demonstrated a role for each protein in maintaining cell morphology. Furthermore, we demonstrate that RhoC expression and activation is required for directed migration and invasion, while Rac1 expression and activation is required for tumor cell diapedesis. Inhibition of RhoA expression produced a slight increase in invasion and tumor cell diapedesis.
conclusionsIndividual Rho GTPases are required for critical aspects of migration, invasion and tumor cell diapedesis. These data suggest that coordinated activation of individual Rho proteins is required for cells to successfully complete the extravasation process; a key step in distant metastasis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.