ArticleThe Journal of biological chemistry2008
A small stem loop element directs internal initiation of the URE2 internal ribosome entry site in Saccharomyces cerevisiae.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 31 citations in OpenAlex.
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- Long-read single-molecule RNA structure sequencing using nanopore.Nucleic acids research · 2022Article
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- A Retrospective on eIF2A-and Not the Alpha Subunit of eIF2.International journal of molecular sciences · 2020Review
- Melting temperature highlights functionally important RNA structure and sequence elements in yeast mRNA coding regions.Nucleic acids research · 2017Article
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- Signature gene expression reveals novel clues to the molecular mechanisms of dimorphic transition in Penicillium marneffei.PLoS genetics · 2014Article
- Multiple inputs control sulfur-containing amino acid synthesis in Saccharomyces cerevisiae.Molecular biology of the cell · 2014Article
- The unfolded protein response in the protozoan parasite Toxoplasma gondii features translational and transcriptional control.Eukaryotic cell · 2013Article
- IRES-mediated translation of the pro-apoptotic Bcl2 family member PUMA.Translation (Austin, Tex.) · 2013Article
- Secondary RNA structure and nucleotide specificity contribute to internal initiation mediated by the human tau 5' leader.RNA biology · 2012Article
- Review
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- Understanding the transcriptome through RNA structure.Nature reviews. Genetics · 2011Review
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- Alternative ways to think about cellular internal ribosome entry.The Journal of biological chemistry · 2010Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Internal initiation of translation is the process of beginning protein synthesis independent of the m(7)G cap structure at the 5'-end of an mRNA molecule. We have previously shown that the URE2 mRNA in the yeast Saccharomyces cerevisiae contains an internal ribosome entry site (IRES) whose activity is suppressed by eukaryotic initiation factor 2A (eIF2A; YGR054W). In this study, the minimal sequence required to efficiently direct internal initiation was determined using a system that abrogates cap-dependent scanning of the 40 S ribosomal subunit in both wild-type and eIF2A knock-out cells. Subsequently, secondary structural elements within the minimal sequence were determined by probing with RNases T1 and V1 and the small molecule diethylpyrocarbonate. It was found that the URE2 minimal IRES comprises a 104 nucleotide A-rich stem loop element encompassing the internal AUG codon. Interestingly, the internal AUG seems to be involved in base-pairing interactions that would theoretically hamper its ability to interact with incoming initiator tRNA molecules. Furthermore, none of the truncations used to identify the minimal IRES element were capable of abrogating the suppressive effect of eIF2A. Our data provide the first insight into the RNA structural requirements of the yeast translational machinery for cap-independent initiation of protein synthesis.
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