ArticlePlanta2008
Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation.
Article in Planta, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- From pollen precursor to totipotent embryo: a model system for plant cell developmental plasticity.Plant cell reports · 2026Review
- Molecular Insights into Microspore Embryogenesis: Transcriptomic Dynamics in Barley and Wheat.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Exogenous Glutathione Promotes the Proliferation ofPlants (Basel, Switzerland) · 2022Article
- Glutathione provides antioxidative defence and promotes microspore-derived embryo development in isolated microspore cultures of triticale (× Triticosecale Wittm.).Plant cell reports · 2019Article
- Redox Balance-DDR-miRNA Triangle: Relevance in Genome Stability and Stress Responses in Plants.Frontiers in plant science · 2019Review
- Overexpression of the brassinosteroid biosynthetic gene DWF4 in Brassica napus simultaneously increases seed yield and stress tolerance.Scientific reports · 2016Article
- ROS Homeostasis Regulates Somatic Embryogenesis via the Regulation of Auxin Signaling in Cotton.Molecular & cellular proteomics : MCP · 2016Article
- Effects of altered expression ofJournal, genetic engineering & biotechnology · 2016Article
- Vitamin C deficiency improves somatic embryo development through distinct gene regulatory networks in Arabidopsis.Journal of experimental botany · 2014Article
- Microspore embryogenesis in wheat: new marker genes for early, middle and late stages of embryo development.Plant reproduction · 2013Article
- Microspore embryogenesis: establishment of embryo identity and pattern in culture.Plant reproduction · 2013Review
- Article
- Transcriptome analysis of nodes and buds from high and low tillering switchgrass inbred lines.PloS one · 2013Article
- Androgenesis in recalcitrant solanaceous crops.Plant cell reports · 2011Review
- Modulation of embryo-forming capacity in culture through the expression of Brassica genes involved in the regulation of the shoot apical meristem.Journal of experimental botany · 2010Article
- Identification of novel genes potentially involved in somatic embryogenesis in chicory (Cichorium intybus L.).BMC plant biology · 2010Article
- Depletion of cellular brassinolide decreases embryo production and disrupts the architecture of the apical meristems in Brassica napus microspore-derived embryos.Journal of experimental botany · 2010Article
- Patterns of differential gene expression in Brassica napus cultivars infected with Sclerotinia sclerotiorum.Molecular plant pathology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Applications of buthionine sulfoximine (BSO), an inhibitor of GSH (reduced glutathione), which switches the cellular glutathione pool towards the oxidized form GSSG, positively influences embryo quality by improving the structure of the shoot apical meristem and promoting embryo maturation, both of which improve the post-embryonic performance of the embryos. To investigate the mechanisms underlying BSO-mediated improvement in embryo quality the transcript profiles of developing Brassica napus microspore-derived embryos cultured in the absence (control) or presence of BSO were analyzed using a 15,000-element B. napus oligo microarray. BSO applications induced major changes in transcript accumulation patterns, especially during the late phases of embryogenesis. BSO affected the transcription and activities of key enzymes involved in ascorbate metabolism, which resulted in major fluctuations in cellular ascorbate levels. These changes were related to morphological characteristics of the embryos and their post-embryonic performance. BSO applications also activated many genes controlling meristem formation and function, including ZWILLE, SHOOTMERISTEMLESS, and ARGONAUTE 1. Increased expression of these genes may contribute to the improved structural quality of the shoot poles observed in the presence of BSO. Compared to their control counterparts, middle- and late-stage BSO-treated embryos also showed increased accumulation of transcripts associated with the maturation phase of zygotic embryo development, including genes encoding ABA-responsive proteins and storage- and late-embryogenic abundant (LEA) proteins. Overall these transcriptional changes support the observation that the BSO-induced oxidized glutathione redox state allows cultured embryos to reach both morphological and physiological maturity, which in turn guarantees successful regeneration and enhanced post-embryonic growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.