ArticleProceedings of the National Academy of Sciences of the United States of America2008
Cis- and trans-splicing of mRNAs mediated by tRNA sequences in eukaryotic cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- tRNA as an assembly chaperone for a macromolecular transcription-processing complex.Nature structural & molecular biology · 2025Article
- Introns and Their Therapeutic Applications in Biomedical Researches.Iranian journal of biotechnology · 2023Review
- Alternative and aberrant splicing of human endogenous retroviruses in cancer. What about head and neck? -A mini review.Frontiers in oncology · 2022Review
- Archaeal tRNA-Splicing Endonuclease as an Effector for RNA Recombination and Novel Trans-Splicing Pathways in Eukaryotes.Journal of fungi (Basel, Switzerland) · 2021Article
- ChiTaRS 5.0: the comprehensive database of chimeric transcripts matched with druggable fusions and 3D chromatin maps.Nucleic acids research · 2020Article
- AtFusionDB: a database of fusion transcripts in Arabidopsis thaliana.Database : the journal of biological databases and curation · 2019Article
- ChiTaRS-3.1-the enhanced chimeric transcripts and RNA-seq database matched with protein-protein interactions.Nucleic acids research · 2017Article
- Analysis of random PCR-originated mutants of the yeast Ste2 and Ste3 receptors.MicrobiologyOpen · 2016Article
- Evolutionary Insights into RNA trans-Splicing in Vertebrates.Genome biology and evolution · 2016Review
- InFusion: Advancing Discovery of Fusion Genes and Chimeric Transcripts from Deep RNA-Sequencing Data.PloS one · 2016Article
- ChiTaRS 2.1--an improved database of the chimeric transcripts and RNA-seq data with novel sense-antisense chimeric RNA transcripts.Nucleic acids research · 2015Article
- ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data.Nucleic acids research · 2013Article
- Identification and analysis of pig chimeric mRNAs using RNA sequencing data.BMC genomics · 2012Article
- Yeast pheromone receptor genes STE2 and STE3 are differently regulated at the transcription and polyadenylation level.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- tRNA biology charges to the front.Genes & development · 2010Review
- Splicing of mRNA mediated by tRNA sequences in mouse cells.RNA (New York, N.Y.) · 2009Article
- A transcriptional sketch of a primary human breast cancer by 454 deep sequencing.BMC genomics · 2009Article
- Long-distance splicing.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
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3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The formation of chimeric mRNAs is a strategy used by human cells to increase the complexity of their proteome, as revealed by the ENCODE project. Here, we use Saccharomyces cerevisiae to show a way by which trans-spliced mRNAs can be generated. We demonstrate that a pretRNA inserted into a premRNA context directs the splicing reaction precisely to the sites of the tRNA intron. A suppressor pretRNA gene was inserted, in cis, into the sequence encoding the third cytoplasmic loop of the Ste2 or Ste3 G protein-coupled receptor. The hybrid RNAs are spliced at the specific pretRNA splicing sites, releasing both functional tRNAs that suppress nonsense mutations and translatable mRNAs that activate the signal transduction pathway. The RNA molecules extracted from yeast cells were amplified by RT-PCR, and their sequences were determined, confirming the identity of the splice junctions. We then constructed two fusions between the premRNA sequence (STE2 or STE3) and the 5'- or 3'-pretRNA half, so that the two hybrid RNAs can associate with each other, in trans, through their tRNA halves. Splicing occurs at the predicted pretRNA sites, producing a chimeric STE3-STE2 receptor mRNA. RNA trans-splicing mediated by tRNA sequences, therefore, is a mechanism capable of producing new kinds of RNAs, which could code for novel proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.