ArticleEukaryotic cell2008
Functional characterization of CgCTR2, a putative vacuole copper transporter that is involved in germination and pathogenicity in Colletotrichum gloeosporioides.
Article in Eukaryotic cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 32 citations in OpenAlex.
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- Mango anthracnose disease: the current situation and direction for future research.Frontiers in microbiology · 2023Review
- TheFrontiers in plant science · 2019Article
- Cell-surface copper transporters and superoxide dismutase 1 are essential for outgrowth during fungal spore germination.The Journal of biological chemistry · 2017Article
- Plant Pathogenic Fungi.Microbiology spectrum · 2017Review
- Pleiotropic effects of the vacuolar ABC transporter MLT1 of Candida albicans on cell function and virulence.The Biochemical journal · 2016Article
- The copper transporter 1 (CTR1) is required to maintain the stability of copper transporter 2 (CTR2).Metallomics : integrated biometal science · 2015Article
- Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous ascomyceteMicrobial cell (Graz, Austria) · 2014Article
- Copper transporter 2 regulates intracellular copper and sensitivity to cisplatin.Metallomics : integrated biometal science · 2014Article
- Genome-wide analysis of copper, iron and zinc transporters in the arbuscular mycorrhizal fungus Rhizophagus irregularis.Frontiers in plant science · 2014Article
- Brachypodium distachyon as a model system for studies of copper transport in cereal crops.Frontiers in plant science · 2014Article
- Molecular and functional analyses of COPT/Ctr-type copper transporter-like gene family in rice.BMC plant biology · 2011Article
- Regulation of Cisplatin cytotoxicity by cu influx transporters.Metal-based drugs · 2010Article
- CgOpt1, a putative oligopeptide transporter from Colletotrichum gloeosporioides that is involved in responses to auxin and pathogenicity.BMC microbiology · 2009Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Copper is a cofactor and transition metal involved in redox reactions that are essential in all eukaryotes. Here, we report that a vacuolar copper transporter that is highly expressed in resting spores is involved in germination and pathogenicity in the plant pathogen Colletotrichum gloeosporioides. A screen of C. gloeosporioides transformants obtained by means of a promoterless green fluorescent protein (GFP) construct led to the identification of transformant N159 in which GFP signal was observed in spores. The transforming vector was inserted 70 bp upstream of a putative gene with homology to the Saccharomyces cerevisiae vacuolar copper transporter gene CTR2. The C. gloeosporioides CTR2 (CgCTR2) gene fully complemented growth defects of yeast ctr2Delta mutants, and a CgCTR2-cyan fluorescent protein (CFP) fusion protein accumulated in vacuole membranes, confirming the function of the protein as a vacuolar copper transporter. Expression analysis indicated that CgCTR2 transcript is abundant in resting conidia and during germination in rich medium and downregulated during "pathogenic" germination and the early stages of plant infection. CgCTR2 overexpression and silencing mutants were generated and characterized. The Cgctr2 mutants had markedly reduced Cu superoxide dismutase (SOD) activity, suggesting that CgCTR2 is important in providing copper to copper-dependent cytosolic activities. The Cgctr2-silenced mutants had increased sensitivity to H2O2 and reduced germination rates. The mutants were also less virulent to plants, but they did not display any defects in appressorium formation and penetration efficiency. An external copper supply compensated for the hypersensitivity to H2O2 but not for the germination and pathogenicity defects of the mutants. Similarly, overexpression of CgCTR2 enhanced resistance to H2O2 but had no effect on germination or pathogenicity. Our results show that copper is necessary for optimal germination and pathogenicity and that CgCTR2 is involved in regulating cellular copper balance during these processes.
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