ArticleBiophysical journal2008
Biophysical characterization of the unstructured cytoplasmic domain of the human neuronal adhesion protein neuroligin 3.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 53 citations in OpenAlex.
- Semi-synthesis of the C-terminal region ofRSC chemical biology · 2026Article
- Hydrodynamic Radii of Intrinsically Disordered Proteins: Fast Prediction by Minimum Dissipation Approximation and Experimental Validation.The journal of physical chemistry letters · 2024Article
- Diversity of hydrodynamic radii of intrinsically disordered proteins.European biophysics journal : EBJ · 2023Article
- Functional Implications of Dynamic Structures of Intrinsically Disordered Proteins Revealed by High-Speed AFM Imaging.Biomolecules · 2022Review
- Improved predictions of phase behaviour of intrinsically disordered proteins by tuning the interaction range.Open research Europe · 2022Article
- Integrative structural dynamics probing of the conformational heterogeneity in synaptosomal-associated protein 25.Cell reports. Physical science · 2021Article
- Accurate model of liquid-liquid phase behavior of intrinsically disordered proteins from optimization of single-chain properties.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain.Biophysical journal · 2020Article
- Predicting Conformational Properties of Intrinsically Disordered Proteins from Sequence.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Bacterial functional amyloids: Order from disorder.Biochimica et biophysica acta. Proteins and proteomics · 2019Review
- BASILIScan: a tool for high-throughput analysis of intrinsic disorder patterns in homologous proteins.BMC genomics · 2018Article
- Resolution of Two Sub-Populations of Conformers and Their Individual Dynamics by Time Resolved Ensemble Level FRET Measurements.PloS one · 2015Article
- VirtualSpectrum, a tool for simulating peak list for multi-dimensional NMR spectra.Journal of biomolecular NMR · 2014Article
- Tracking the origin and divergence of cholinesterases and neuroligins: the evolution of synaptic proteins.Journal of molecular neuroscience : MN · 2014Article
- Describing sequence-ensemble relationships for intrinsically disordered proteins.The Biochemical journal · 2013Review
- Backbone and side chain NMR assignments for the intrinsically disordered cytoplasmic domain of human neuroligin-3.Biomolecular NMR assignments · 2012Article
- Article
- Context-dependent resistance to proteolysis of intrinsically disordered proteins.Protein science : a publication of the Protein Society · 2011Review
- Beyond the random coil: stochastic conformational switching in intrinsically disordered proteins.Structure (London, England : 1993) · 2011Article
- ncIDP-assign: a SPARKY extension for the effective NMR assignment of intrinsically disordered proteins.Bioinformatics (Oxford, England) · 2011Article
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Authors and funding
11 authors at 4 institutions in 4 countries.
Funding
Abstract
Cholinesterase-like adhesion molecules (CLAMs) are a family of neuronal cell adhesion molecules with important roles in synaptogenesis, and in maintaining structural and functional integrity of the nervous system. Our earlier study on the cytoplasmic domain of one of these CLAMs, the Drosophila protein, gliotactin, showed that it is intrinsically unstructured in vitro. Bioinformatic analysis suggested that the cytoplasmic domains of other CLAMs are also intrinsically unstructured, even though they bear no sequence homology to each other or to any known protein. In this study, we overexpress and purify the cytoplasmic domain of human neuroligin 3, notwithstanding its high sensitivity to the Escherichia coli endogenous proteases that cause its rapid degradation. Using bioinformatic analysis, sensitivity to proteases, size exclusion chromatography, fluorescence correlation spectroscopy, analytical ultracentrifugation, small angle x-ray scattering, circular dichroism, electron spin resonance, and nuclear magnetic resonance, we show that the cytoplasmic domain of human neuroligin 3 is intrinsically unstructured. However, several of these techniques indicate that it is not fully extended, but becomes significantly more extended under denaturing conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.