ArticleNucleic acids research2008
Ribosomal RNAs are tolerant toward genetic insertions: evolutionary origin of the expansion segments.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Varying strength of selection contributes to the intragenomic diversity of rRNA genes.Nature communications · 2022Article
- Peptide Bond Formation between Aminoacyl-Minihelices by a Scaffold Derived from the Peptidyl Transferase Center.Life (Basel, Switzerland) · 2022Article
- Cryo-electron microscopy visualization of a large insertion in the 5S ribosomal RNA of the extremely halophilic archaeon Halococcus morrhuae.FEBS open bio · 2020Article
- Piece by piece: Building a ribozyme.The Journal of biological chemistry · 2020Review
- The Jigsaw Puzzle of mRNA Translation Initiation in Eukaryotes: A Decade of Structures Unraveling the Mechanics of the Process.Annual review of biophysics · 2018Article
- The Expansion Segments of 28S Ribosomal RNA Extensively Match Human Messenger RNAs.Frontiers in genetics · 2018Article
- Size-variable zone in V3 region of 16S rRNA.RNA biology · 2017Article
- One step engineering of the small-subunit ribosomal RNA using CRISPR/Cas9.Scientific reports · 2016Article
- Ribosome Subunit Stapling for Orthogonal Translation inAngewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2015Article
- Ribosome Subunit Stapling for Orthogonal Translation in E. coli.Angewandte Chemie (International ed. in English) · 2015Article
- Computing the origin and evolution of the ribosome from its structure - Uncovering processes of macromolecular accretion benefiting synthetic biology.Computational and structural biotechnology journal · 2015Review
- Ribosomal RNA gene transcription in trypanosomes.Parasitology research · 2014Review
- Article
- The structure and function of the eukaryotic ribosome.Cold Spring Harbor perspectives in biology · 2012Review
- Ribosome distribution in HeLa cells during the cell cycle.PloS one · 2012Article
- Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Origin and evolution of the ribosome.Cold Spring Harbor perspectives in biology · 2010Review
- Evolutionary triplet models of structured RNA.PLoS computational biology · 2009Article
- Article
- The GA-minor submotif as a case study of RNA modularity, prediction, and design.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribosomal RNAs (rRNAs), assisted by ribosomal proteins, form the basic structure of the ribosome, and play critical roles in protein synthesis. Compared to prokaryotic ribosomes, eukaryotic ribosomes contain elongated rRNAs with several expansion segments and larger numbers of ribosomal proteins. To investigate architectural evolution and functional capability of rRNAs, we employed a Tn5 transposon system to develop a systematic genetic insertion of an RNA segment 31 nt in length into Escherichia coli rRNAs. From the plasmid library harboring a single rRNA operon containing random insertions, we isolated surviving clones bearing rRNAs with functional insertions that enabled rescue of the E. coli strain (Delta7rrn) in which all chromosomal rRNA operons were depleted. We identified 51 sites with functional insertions, 16 sites in 16S rRNA and 35 sites in 23S rRNA, revealing the architecture of E. coli rRNAs to be substantially flexible. Most of the insertion sites show clear tendency to coincide with the regions of the expansion segments found in eukaryotic rRNAs, implying that eukaryotic rRNAs evolved from prokaryotic rRNAs suffering genetic insertions and selections.
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