ArticleBMC microbiology2008
Identification of new genes in Sinorhizobium meliloti using the Genome Sequencer FLX system.
Article in BMC microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Article
- Molecular techniques in the biotechnological fight against halogenated compounds in anoxic environments.Microbial biotechnology · 2012Review
- Efficient and robust RNA-seq process for cultured bacteria and complex community transcriptomes.Genome biology · 2012Article
- An ABC-type cobalt transport system is essential for growth of Sinorhizobium meliloti at trace metal concentrations.Journal of bacteriology · 2011Article
- Transcriptomic signatures of ash (Fraxinus spp.) phloem.PloS one · 2011Article
- Validation of two ribosomal RNA removal methods for microbial metatranscriptomics.Nature methods · 2010Article
- Studying bacterial transcriptomes using RNA-seq.Current opinion in microbiology · 2010Review
- Population-level transcriptome sequencing of nonmodel organisms Erynnis propertius and Papilio zelicaon.BMC genomics · 2010Article
- A genome-wide survey of sRNAs in the symbiotic nitrogen-fixing alpha-proteobacterium Sinorhizobium meliloti.BMC genomics · 2010Article
- Dual control of Sinorhizobium meliloti RpoE2 sigma factor activity by two PhyR-type two-component response regulators.Journal of bacteriology · 2010Article
- RNA-seq: from technology to biology.Cellular and molecular life sciences : CMLS · 2010Review
- Prokaryotic transcriptomics: a new view on regulation, physiology and pathogenicity.Nature reviews. Genetics · 2010Review
- Article
- Transcriptome sequencing of the Microarray Quality Control (MAQC) RNA reference samples using next generation sequencing.BMC genomics · 2009Article
- Article
- A portal for rhizobial genomes: RhizoGATE integrates a Sinorhizobium meliloti genome annotation update with postgenome data.Journal of biotechnology · 2009Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSinorhizobium meliloti is an agriculturally important model symbiont. There is an ongoing need to update and improve its genome annotation. In this study, we used a high-throughput pyrosequencing approach to sequence the transcriptome of S. meliloti, and search for new bacterial genes missed in the previous genome annotation. This is the first report of sequencing a bacterial transcriptome using the pyrosequencing technology.
resultsOur pilot sequencing run generated 19,005 reads with an average length of 136 nucleotides per read. From these data, we identified 20 new genes. These new gene transcripts were confirmed by RT-PCR and their possible functions were analyzed.
conclusionOur results indicate that high-throughput sequence analysis of bacterial transcriptomes is feasible and next-generation sequencing technologies will greatly facilitate the discovery of new genes and improve genome annotation.
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