ArticleAntonie van Leeuwenhoek2008
recA mediated spontaneous deletions of the icaADBC operon of clinical Staphylococcus epidermidis isolates: a new mechanism of phenotypic variations.
Article in Antonie van Leeuwenhoek, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 23 citations in OpenAlex.
- Metabolic and regulatory adaptations of Staphylococcus epidermidis to host stresses.FEMS microbiology reviews · 2026Review
- An investigation on anti-biofilm potential of Aegle marmelos fruit extract against multi-drug-resistant Staphylococcus aureus.BMC complementary medicine and therapies · 2025Article
- Effect of Ultraviolet Light on the Expression ofArchives of Razi Institute · 2021Article
- Influence of Preparation Procedure on Physicochemical and Antibacterial Properties of Titanate Nanotubes Modified with Silver.Nanomaterials (Basel, Switzerland) · 2019Article
- The small non-coding RNA RsaE influences extracellular matrix composition in Staphylococcus epidermidis biofilm communities.PLoS pathogens · 2019Article
- Prevalence of Panton-Valentine Leukocidin Gene among Community AcquiredJournal of pathogens · 2018Article
- Biofilm formation by Staphylococcus hominis strains isolated from human clinical specimens.Folia microbiologica · 2015Article
- Effectiveness of Cinnamon Oil Coating on K-wire as an Antimicrobial Agent against Staphylococcus Epidermidis.Malaysian orthopaedic journal · 2013Article
- Bacterial cell surface heterogeneity: a pathogen's disguise.PLoS pathogens · 2012Article
- Survival of adhering staphylococci during exposure to a quaternary ammonium compound evaluated by using atomic force microscopy imaging.Antimicrobial agents and chemotherapy · 2011Article
- Article
- Effect of cinnamon oil on icaA expression and biofilm formation by Staphylococcus epidermidis.Applied and environmental microbiology · 2009Article
- Biofilm formation by Staphylococcus haemolyticus.Journal of clinical microbiology · 2009Article
- The presence of icaADBC is detrimental to the colonization of human skin by Staphylococcus epidermidis.Applied and environmental microbiology · 2008Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phenotypic variation of Staphylococcus epidermidis involving the slime related ica operon results in heterogeneity in surface characteristics of individual bacteria in axenic cultures. Five clinical S. epidermidis isolates demonstrated phenotypic variation, i.e. both black and red colonies on Congo Red agar. Black colonies displayed bi-modal electrophoretic mobility distributions at pH 2, but such phenotypic variation was absent in red colonies of the same strain as well as in control strains without phenotypic variation. All red colonies had lost ica and the ability to form biofilms, in contrast to black colonies of the same strain. Real time PCR targeting icaA indicated a reduction in gene copy number within cultures exhibiting phenotypic variation, which correlated with phenotypic variations in biofilm formation and electrophoretic mobility distribution of cells within a culture. Loss of ica was irreversible and independent of the mobile element IS256. Instead, in high frequency switching strains, spontaneous mutations in lexA were found which resulted in deregulation of recA expression, as shown by real time PCR. RecA is involved in genetic deletions and rearrangements and we postulate a model representing a new mechanism of phenotypic variation in clinical isolates of S. epidermidis. This is the first report of S. epidermidis strains irreversibly switching from biofilm-positive to biofilm-negative phenotype by spontaneous deletion of icaADBC.
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