ArticleJournal of immunology (Baltimore, Md. : 1950)2008
The Downstream Transcriptional Enhancer, Ed, positively regulates mouse Ig kappa gene expression and somatic hypermutation.
Article in Journal of immunology (Baltimore, Md. : 1950), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- Enhancers within the Ig V Gene Region Orchestrate Chromatin Topology and Regulate V Gene Rearrangement Frequency to Shape the B Cell Receptor Repertoire Specificities.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Article
- ERCC6L2 promotes DNA orientation-specific recombination in mammalian cells.Cell research · 2020Article
- REV7 is required for processing AID initiated DNA lesions in activated B cells.Nature communications · 2020Article
- What Targets Somatic Hypermutation to the Immunoglobulin Loci?Viral immunology · 2020Review
- A B-Cell-Specific Enhancer Orchestrates Nuclear Architecture to Generate a Diverse Antigen Receptor Repertoire.Molecular cell · 2019Article
- YY1 binds to the E3' enhancer and inhibits the expression of the immunoglobulin κ gene via epigenetic modifications.Immunology · 2018Article
- Enhancer talk.Epigenomics · 2018Review
- Efficient AID targeting of switch regions is not sufficient for optimal class switch recombination.Nature communications · 2015Article
- A major deletion in the Vκ-Jκ intervening region results in hyperelevated transcription of proximal Vκ genes and a severely restricted repertoire.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- A novel pax5-binding regulatory element in the igκ locus.Frontiers in immunology · 2014Article
- The IgH 3' regulatory region controls somatic hypermutation in germinal center B cells.The Journal of experimental medicine · 2013Article
- Loss of an Igκ gene enhancer in mature B cells results in rapid gene silencing and partial reversible dedifferentiation.Molecular and cellular biology · 2013Article
- A new hypersensitive site, HS10, and the enhancers, E3' and Ed, differentially regulate Igκ gene expression.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Shadow enhancers: frequently asked questions about distributed cis-regulatory information and enhancer redundancy.BioEssays : news and reviews in molecular, cellular and developmental biology · 2012Article
- A multifunctional element in the mouse Igκ locus that specifies repertoire and Ig loci subnuclear location.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Article
- Targeting of AID-mediated sequence diversification to immunoglobulin genes.Current opinion in immunology · 2011Review
- The Igκ gene enhancers, E3' and Ed, are essential for triggering transcription.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- AID and somatic hypermutation.Advances in immunology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The mouse Igkappa locus has three known transcriptional enhancers: the matrix association region/intronic enhancer, the 3' enhancer (E3'), and the further downstream enhancer (Ed). Previous studies have shown that both matrix association region/intronic and E3' enhancers are required for maximal gene rearrangement of the locus, and that E3' is also required for maximal expression and somatic hypermutation (SHM). To functionally elucidate Ed in vivo, we generated knockout mice with a targeted germline deletion of Ed. Ed deleted homozygous mice (Ed-/-) have moderately reduced numbers of Igkappa expressing B cells and correspondingly increased numbers of Iglambda expressing B cells in spleen. Ed-/- mice also have decreased Igkappa mRNA expression in resting and T cell-dependent activated splenic B cells and reduced Igkappa chains in sera. However, our analysis indicates that Igkappa gene rearrangement is normal in Ed-/- mice. In addition, our results show that Ed-/- mice exhibit reduced SHM in the Igkappa gene J-C intronic region in germinal center B cells from Peyer's patches. We conclude that Ed positively regulates Igkappa gene expression and SHM, but not gene rearrangement.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.