ArticleMolecular & cellular proteomics : MCP2008
A proteomics approach for identification of single strand DNA-binding proteins involved in transcriptional regulation of mouse mu opioid receptor gene.
Article in Molecular & cellular proteomics : MCP, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Heroin addiction modulates transcription factor binding in regulatory regions of the human putamen.Scientific reports · 2026Article
- Comparative analysis of retroviral Gag-host cell interactions: focus on the nuclear interactome.bioRxiv : the preprint server for biology · 2024Article
- HNF4A-AS1 inhibits the progression of hepatocellular carcinoma by promoting the ubiquitin-modulated degradation of PCBP2 and suppressing the stability of ARG2 mRNA.International journal of biological sciences · 2024Article
- Heterogeneous nuclear ribonucleoprotein K inhibits heat shock-induced transcriptional activity of heat shock factor 1.The Journal of biological chemistry · 2017Article
- Differential regulation of mouse and human Mu opioid receptor gene depends on the single stranded DNA structure of its promoter and α-complex protein 1.Biomedical reports · 2017Article
- Post-Transcriptional Regulation of the Human Mu-Opioid Receptor (MOR) by Morphine-Induced RNA Binding Proteins hnRNP K and PCBP1.Journal of cellular physiology · 2017Article
- Article
- The hnRNP family: insights into their role in health and disease.Human genetics · 2016Review
- RNA-binding proteins, neural development and the addictions.Genes, brain, and behavior · 2016Review
- Transcriptional regulation of heterogeneous nuclear ribonucleoprotein K gene expression.Biochimie · 2015Article
- Morphine drives internal ribosome entry site-mediated hnRNP K translation in neurons through opioid receptor-dependent signaling.Nucleic acids research · 2014Article
- Vimentin interacts with the 5'-untranslated region of mouse mu opioid receptor (MOR) and is required for post-transcriptional regulation.RNA biology · 2013Article
- Mu opioids and their receptors: evolution of a concept.Pharmacological reviews · 2013Review
- Epigenetics of µ-opioid receptors: intersection with HIV-1 infection of the central nervous system.Journal of cellular physiology · 2012Review
- Post-transcriptional regulation of mu-opioid receptor: role of the RNA-binding proteins heterogeneous nuclear ribonucleoprotein H1 and F.Cellular and molecular life sciences : CMLS · 2012Article
- p38 mitogen-activated protein kinase and PI3-kinase are involved in up-regulation of mu opioid receptor transcription induced by cycloheximide.Journal of neurochemistry · 2011Article
- The cyclin-dependent kinase inhibitor p21 is regulated by RNA-binding protein PCBP4 via mRNA stability.Nucleic acids research · 2011Article
- Up-regulation of the mu-opioid receptor gene is mediated through chromatin remodeling and transcriptional factors in differentiated neuronal cells.Molecular pharmacology · 2010Article
- Differential use of an in-frame translation initiation codon regulates human mu opioid receptor (OPRM1).Cellular and molecular life sciences : CMLS · 2009Article
- Poly(C)-binding proteins as transcriptional regulators of gene expression.Biochemical and biophysical research communications · 2009Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The pharmacological actions of morphine and morphine-like drugs such as heroin are mediated primarily through the mu opioid receptor. Previously a single strand DNA element of the mouse mu opioid receptor gene (Oprm1) proximal promoter was found to be important for regulating Oprm1 in neuronal cells. To identify proteins binding to the single strand DNA element as potential regulators for Oprm1, affinity column chromatography with the single strand DNA element was performed using neuroblastoma NS20Y cells followed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. We identified five poly(C)-binding proteins: heterogeneous nuclear ribonucleoprotein (hnRNP) K, alpha-complex proteins (alphaCP) alphaCP1, alphaCP2, alphaCP2-KL, and alphaCP3. Binding of these proteins to the single strand DNA element of Oprm1 was sequence-specific as confirmed by supershift assays. In cotransfection studies, hnRNP K, alphaCP1, alphaCP2, and alphaCP2-KL activated the Oprm1 promoter activity, whereas alphaCP3 acted as a repressor. Ectopic expression of hnRNP K, alphaCP1, alphaCP2, and alphaCP2-KL also led to activation of the endogenous Oprm1 transcripts, and alphaCP3 repressed endogenous Oprm1 transcripts. We demonstrate novel roles as transcriptional regulators in Oprm1 regulation for hnRNP K and alphaCP binding to the single strand DNA element.
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