ArticleMolecular microbiology2008
Large-scale transposon mutagenesis of Mycoplasma pulmonis.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed.
- A highly conserved tRNA modification contributes toMicrobiology spectrum · 2024Article
- Improved transformation efficiency inMicrobiology spectrum · 2023Article
- Gene editing tools for mycoplasmas: references and future directions for efficient genome manipulation.Frontiers in microbiology · 2023Review
- Non-essential ribosomal proteins in bacteria and archaea identified using COGs.Journal of bacteriology · 2021Article
- DEG 15, an update of the Database of Essential Genes that includes built-in analysis tools.Nucleic acids research · 2021Article
- An emerging role for cyclic dinucleotide phosphodiesterase and nanoRNase activities in Mycoplasma bovis: Securing survival in cell culture.PLoS pathogens · 2020Article
- ePath: an online database towards comprehensive essential gene annotation for prokaryotes.Scientific reports · 2019Article
- SynMyco transposon: engineering transposon vectors for efficient transformation of minimal genomes.DNA research : an international journal for rapid publication of reports on genes and genomes · 2019Article
- Attenuation of a PathogenicmSphere · 2019Article
- The Tyrosine-Autokinase UbK Is Required for Proper Cell Growth and Cell Morphology ofFrontiers in microbiology · 2019Article
- Large-Scale Analysis of theFrontiers in microbiology · 2019Article
- Metabolic models and gene essentiality data reveal essential and conserved metabolism in prokaryotes.PLoS computational biology · 2018Article
- Minimal Cells-Real and Imagined.Cold Spring Harbor perspectives in biology · 2017Review
- Identification of cellular targets of a series of boron heterocycles using TIPA II-A sensitive target identification platform.Bioorganic & medicinal chemistry · 2016Article
- Essentiality of threonylcarbamoyladenosine (t(6)A), a universal tRNA modification, in bacteria.Molecular microbiology · 2015Article
- Uncovering major genomic features of essential genes in Bacteria and a methanogenic Archaea.The FEBS journal · 2015Article
- Cross-talk between two nucleotide-signaling pathways in Staphylococcus aureus.The Journal of biological chemistry · 2015Article
- Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium.Molecular systems biology · 2015Article
- General N-and O-Linked Glycosylation of Lipoproteins in Mycoplasmas and Role of Exogenous Oligosaccharide.PloS one · 2015Article
- Likelihood-based gene annotations for gap filling and quality assessment in genome-scale metabolic models.PLoS computational biology · 2014Article
14 more citing papers are in PubMed but not listed here.
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6 authors.
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Abstract
To obtain mutants for the study of the basic biology and pathogenic mechanisms of mycoplasmas, the insertion site of transposon Tn4001T was determined for 1700 members of a library of Mycoplasma pulmonis mutants. After evaluating several criteria for gene disruption, we concluded that 321 of the 782 protein coding regions were inactivated. The dispensable and essential genes of M. pulmonis were compared with those reported for Mycoplasma genitalium and Bacillus subtilis. Perhaps the most surprising result of the current study was that unlike other bacteria, ribosomal proteins S18 and L28 were dispensable. Carbohydrate transport and the susceptibility of selected mutants to UV irradiation were examined to assess whether active transposition of Tn4001T within the genome would confound phenotypic analysis. In contrast to earlier reports suggesting that mycoplasmas were limited in their DNA repair machinery, mutations in recA, uvrA, uvrB and uvrC resulted in a DNA-repair deficient phenotype. A mutant with a defect in transport of N-acetylglucosamine was identified.
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