ArticleMolecular microbiology2008
YscU cleavage and the assembly of Yersinia type III secretion machine complexes.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 50 citations in OpenAlex.
- Orf1B controls secretion of T3SS proteins and contributes to Edwardsiella piscicida adhesion to epithelial cells.Veterinary research · 2022Article
- The substrate specificity switch FlhB assembles onto the export gate to regulate type three secretion.Nature communications · 2020Article
- Article
- HrcU and HrpP are pathogenicity factors in the fire blight pathogen Erwinia amylovora required for the type III secretion of DspA/E.BMC microbiology · 2016Article
- YscU/FlhB of Yersinia pseudotuberculosis Harbors a C-terminal Type III Secretion Signal.The Journal of biological chemistry · 2015Article
- Article
- Identification of secreted bacterial proteins by noncanonical amino acid tagging.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Bacterial type III secretion systems: specialized nanomachines for protein delivery into target cells.Annual review of microbiology · 2014Review
- Functionally essential interaction between Yersinia YscO and the T3S4 domain of YscP.Journal of bacteriology · 2013Article
- Regulation of the Yersinia type III secretion system: traffic control.Frontiers in cellular and infection microbiology · 2013Review
- A translocator-specific export signal establishes the translocator-effector secretion hierarchy that is important for type III secretion system function.Molecular microbiology · 2012Article
- Expanded roles for multicargo and class 1B effector chaperones in type III secretion.Journal of bacteriology · 2012Review
- Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.Microbiology and molecular biology reviews : MMBR · 2012Review
- Impact of the N-terminal secretor domain on YopD translocator function in Yersinia pseudotuberculosis type III secretion.Journal of bacteriology · 2011Article
- Role of EscU auto-cleavage in promoting type III effector translocation into host cells by enteropathogenic Escherichia coli.BMC microbiology · 2011Article
- Structure-function analysis of the HrpB2-HrcU interaction in the Xanthomonas citri type III secretion system.PloS one · 2011Article
- Secretion of early and late substrates of the type III secretion system from Xanthomonas is controlled by HpaC and the C-terminal domain of HrcU.Molecular microbiology · 2011Article
- Multi-Functional Characteristics of the Pseudomonas aeruginosa Type III Needle-Tip Protein, PcrV; Comparison to Orthologs in other Gram-negative Bacteria.Frontiers in microbiology · 2011Article
- Identification of small-molecule inhibitors of Yersinia pestis Type III secretion system YscN ATPase.PloS one · 2011Article
- Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog).Journal of bacteriology · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
YscU, a component of the Yersinia type III secretion machine, promotes auto-cleavage at asparagine 263 (N263). Mutants with an alanine substitution at yscU codon 263 displayed secretion defects for some substrates (LcrV, YopB and YopD); however, transport of effector proteins into host cells (YopE, YopH, YopM) continued to occur. Two yscU mutations were isolated that, unlike N263A, completely abolished type III secretion; YscU(G127D) promoted auto-cleavage at N263, whereas YscU(G270N) did not. When fused to glutathione S-transferase (Gst), the YscU C-terminal cytoplasmic domain promoted auto-cleavage and Gst-YscU(C) also exerted a dominant-negative phenotype by blocking type III secretion. Gst-YscU(C/N263A) caused a similar blockade and Gst-YscU(C/G270N) reduced secretion. Gst-YscU(C) and Gst-YscU(C/N263A) bound YscL, the regulator of the ATPase YscN, whereas Gst-YscU(C/G270N) did not. When isolated from Yersinia, Gst-YscU(C) and Gst-YscU(C/N263A) associated with YscK-YscL-YscQ; however, Gst-YscU(C/G270N) interacted predominantly with the machine component YscO, but not with YscK-YscL-YscQ. A model is proposed whereby YscU auto-cleavage promotes interaction with YscL and recruitment of ATPase complexes that initiate type III secretion.
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