ArticlePlant cell reports2008
Efficient transformation of wheat by using a mutated rice acetolactate synthase gene as a selectable marker.
Article in Plant cell reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 55 citations in OpenAlex.
- Generating Broad-Spectrum Resistance to ALS-Inhibiting Herbicides in Rice by CRISPR/Cas9-Mediated NHEJ.Rice (New York, N.Y.) · 2025Article
- An optimized transformation protocol for Anthoceros agrestis and three more hornwort species.The Plant journal : for cell and molecular biology · 2023Article
- The Development of Herbicide Resistance Crop Plants Using CRISPR/Cas9-Mediated Gene Editing.Genes · 2021Review
- Seed-specific expression of TaYUC10 significantly increases auxin and protein content in wheat seeds.Plant cell reports · 2021Article
- Article
- A rapid method for detection of mutations induced by CRISPR/Cas9-based genome editing in common wheat.Plant biotechnology (Tokyo, Japan) · 2020Article
- Developing dual herbicide tolerant transgenic rice plants for sustainable weed management.Scientific reports · 2018Article
- A novel rice cytochrome P450 gene, CYP72A31, confers tolerance to acetolactate synthase-inhibiting herbicides in rice and Arabidopsis.Plant physiology · 2014Article
- Article
- The road to micronutrient biofortification of rice: progress and prospects.Frontiers in plant science · 2013Article
- The rice mitochondrial iron transporter is essential for plant growth.Nature communications · 2011Article
- Selecting genetic transformants of indica and indica-derived rice cultivars using bispyribac sodium and a mutated ALS gene.Plant cell reports · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acetolactate synthase (ALS) is a target enzyme for many herbicides, including sulfonylurea and imidazolinone. We investigated the usefulness of a mutated ALS gene of rice, which had double point mutations and encoded an herbicide-resistant form of the enzyme, as a selectable marker for wheat transformation. After the genomic DNA fragment from rice containing the mutated ALS gene was introduced into immature embryos by means of particle bombardment, transgenic plants were efficiently selected with the herbicide bispyribac sodium (BS). Southern blot analysis confirmed that transgenic plants had one to more than ten copies of the transgene in their chromosomes. Adjustment of the BS concentration combined with repeated selection effectively prevented nontransgenic plants from escaping herbicide selection. Measurement of ALS activity indicated that transgenic plants produced an herbicide-resistant form of ALS and therefore had acquired the resistance to BS. This report is the first to describe a selection system for wheat transformation that uses a selectable marker gene of plant origin.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.